Bertera Suzanne, Balamurugan A N, Bottino Rita, He Jing, Trucco Massimo
Division of Immunogenetics, Department of Pediatrics, John G. Rangos Research Center, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, Pittsburgh, PA 15201, USA.
J Transplant. 2012;2012:856386. doi: 10.1155/2012/856386. Epub 2012 Dec 9.
Isolation and transplantation of rodent islets are frequently used as a tool for predicting the behavior of new protocols for islet allotransplants in type 1 diabetes patients. Bovine serum albumin (BSA) is recognized as a protease inhibitor possibly protecting function and viability in islets. For this study, the addition of 0.2% BSA to the isolation protocol resulted in a 30% increase in islet yields while other parameters, such as viability and function, retained high islet quality. In vivo, a minimal mass of 70 BSA treated islets showed their ability to control glycemia levels in diabetic mice by bringing the average blood glucose to 153 ± 13.2 mg/dL compared to 288 ± 22.6 mg/dL without BSA. Our results show that the simple addition of BSA to the isolation protocol constitutes a reliable and reproducible method for increasing islet yield. Also adding BSA to the transplantation medium improves islet function in vivo. The method outlined here can reduce the overall number of animals needed per experiment and also reduce the time and resources needed for islet preparation.
啮齿动物胰岛的分离和移植经常被用作一种工具，用于预测1型糖尿病患者胰岛同种异体移植新方案的效果。牛血清白蛋白（BSA）被认为是一种蛋白酶抑制剂，可能对胰岛的功能和活力起到保护作用。在本研究中，在分离方案中添加0.2%的BSA可使胰岛产量提高30%，而其他参数，如活力和功能，仍保持较高的胰岛质量。在体内，70个经BSA处理的胰岛的最小质量显示出它们能够控制糖尿病小鼠的血糖水平，将平均血糖降至153±13.2mg/dL，而未添加BSA时为288±22.6mg/dL。我们的结果表明，在分离方案中简单添加BSA是一种增加胰岛产量的可靠且可重复的方法。在移植培养基中添加BSA也能改善体内胰岛功能。这里概述的方法可以减少每个实验所需的动物总数，也能减少胰岛制备所需的时间和资源。
