该 Harveyi 生物测定法常用于检测 AI-2 群体感应抑制，但由于海洋基质中归一化不一致，因此存在干扰。
The Vibrio harveyi bioassay used routinely to detect AI-2 quorum sensing inhibition is confounded by inconsistent normalization across marine matrices.
机构信息
Graduate Program in Marine Biology, College of Charleston, Charleston, SC 29412, USA.
出版信息
J Microbiol Methods. 2013 Mar;92(3):250-2. doi: 10.1016/j.mimet.2012.12.023. Epub 2013 Jan 7.
PMID:23305926
Abstract
The Vibrio harveyi autoinducer-2 (AI-2) bioassay is used routinely to screen for inhibition of the AI-2 quorum sensing system. The present study utilizes three well-described bacterial strains to demonstrate that inconsistent normalization across matrices undermines the assay's use in screening marine samples for AI-2 inhibition.
摘要
哈维氏弧菌自动诱导物-2（AI-2）生物测定法被常规用于筛选 AI-2 群体感应系统的抑制作用。本研究利用三种描述明确的细菌菌株证明，基质间不一致的归一化会破坏该测定法在筛选海洋样本中 AI-2 抑制作用的用途。
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