Department of Bioengineering, University of California, San Diego, La Jolla, California 92093-0412, USA.
Otolaryngol Head Neck Surg. 2013 Apr;148(4):576-81. doi: 10.1177/0194599812474228. Epub 2013 Jan 15.
To determine and compare the bending moduli of native and engineered human septal cartilage.
Prospective, basic science.
Research laboratory.
Neocartilage constructs were fabricated from expanded human septal chondrocytes cultured in differentiation medium for 10 weeks. Constructs (n = 10) and native septal cartilage (n = 5) were tested in a 3-point bending apparatus, and the bending moduli were calculated using Euler-Bernoulli beam theory.
All samples were tested successfully and returned to their initial shape after unloading. The bending modulus of engineered constructs (0.32 ± 0.25 MPa, mean ± SD) was 16% of that of native septal cartilage (1.97 ± 1.25 MPa).
Human septal constructs, fabricated from cultured human septal chondrocytes, are more compliant in bending than native human septal tissue. The bending modulus of engineered septal cartilage can be measured, and this modulus provides a useful measure of construct rigidity while undergoing maturation relative to native tissue.
确定并比较天然和工程化的人鼻中隔软骨的弯曲模量。
前瞻性基础科学研究。
研究实验室。
从在分化培养基中培养 10 周的扩增人鼻中隔软骨细胞中制备新软骨构建体。使用三点弯曲装置测试构建体（n = 10）和天然鼻中隔软骨（n = 5），并使用 Euler-Bernoulli 梁理论计算弯曲模量。
所有样品均成功测试，并在卸载后恢复到初始形状。工程化构建体的弯曲模量（0.32 ± 0.25 MPa，均值 ± 标准差）为天然鼻中隔软骨（1.97 ± 1.25 MPa）的 16%。
由培养的人鼻中隔软骨细胞制成的人鼻中隔构建体在弯曲时的顺应性比天然人鼻中隔组织更强。工程化鼻中隔软骨的弯曲模量可以测量，该模量提供了一种有用的衡量成熟过程中构建体刚性的指标，与天然组织相比。
