Kubo Akiharu, Nagao Keisuke, Amagai Masayuki
Department of Dermatology, Keio University, Tokyo, Japan.
Methods Mol Biol. 2013;961:119-27. doi: 10.1007/978-1-62703-227-8_5.
Langerhans cells (LCs) are intraepidermal dendritic cells that extend their dendrites between keratinocytes to form a dense network that covers the entire body. The mammalian epidermis has two diffusion barriers, the stratum corneum and tight junctions (TJs). In their resting state, LCs sit underneath these two barriers. Once activated, LCs elongate their dendrites to dock with, or penetrate through, TJs, which allows them to survey the environment between the two barriers. Here, we describe a method to visualize this dynamic interaction of TJs and LCs in 3D by using mouse ear epidermal sheets.
朗格汉斯细胞（LCs）是表皮内的树突状细胞，其树突在角质形成细胞之间延伸，形成覆盖全身的密集网络。哺乳动物的表皮有两个扩散屏障，即角质层和紧密连接（TJs）。在静止状态下，LCs位于这两个屏障之下。一旦被激活，LCs会延长其树突以与紧密连接对接或穿透紧密连接，从而使其能够监测两个屏障之间的环境。在此，我们描述一种通过使用小鼠耳部表皮片在三维空间中可视化紧密连接与LCs这种动态相互作用的方法。
