Dai X L, Zhou S L, Qiu J, Liu Y F, Hua H
Department of Medical Technology, Yancheng Health Vocational and Technical College, Yancheng City, Jiangsu Province, PR China.
Eur J Gynaecol Oncol. 2012;33(6):633-9.
To investigate the expressions of Fas, NF-kappaB, and vascular endothelial growth factor-C (VEGF-C) in infiltrating ductal carcinoma of the breast and provide scientific basis for early diagnosis and prognosis of breast cancer.
The immunohistochemical technique (SP method) was used to detect expression of Fas, NF-kappaB, and VEGF-C in 137 cases of breast-infiltrating ductal carcinoma, 17 cases of intraductal carcinoma of the breast, and 20 cases of normal breast tissues, and analyze its relationship with clinicopathologic factors of breast cancer and patients' survival rate, as well as the correlation among their expression, clinicopathologic factors, and survival rate.
Fas expression was less commonly detected in infiltrating ductal carcinoma than in intraductal carcinoma and normal tissue. In contrast, both NF-kappaB and VEGF-C were more commonly detected in infiltrating ductal carcinoma than in intraductal carcinoma and normal tissue. Fas expression was correlated with tumor size, histological grade, and clinicopathological stage; NF-kappaB expression was correlated with tumor size, histological grade, lymph node metastasis; VEGF-C expression was correlated with lymph node metastasis and clinical and pathological stages of breast cancer (p < or = 0.05). Spearman rank correlation analysis revealed a negative correlation between Fas expression and both NF-kappaB and VEGF-C expression in infiltrating breast cancer (p < 0.05) Additionally, Kaplan-Meier survival analysis demonstrated that five-year survival was higher for patients with Fas-positive samples but lower for those with VEGF-C-positive samples.
The present results demonstrate that Fas and NF-B play a role in the initiation and development of breast cancer, while VEGF-C appears to promote lymph node metastasis. Thus, these proteins may serve as useful diagnostic and prognostic markers of invasive breast cancer.
探讨Fas、核因子-κB（NF-κB）和血管内皮生长因子C（VEGF-C）在乳腺浸润性导管癌中的表达情况，为乳腺癌的早期诊断和预后评估提供科学依据。
采用免疫组织化学SP法检测137例乳腺浸润性导管癌、17例乳腺导管内癌及20例正常乳腺组织中Fas、NF-κB和VEGF-C的表达，分析其与乳腺癌临床病理因素及患者生存率的关系，以及它们之间表达的相关性、临床病理因素与生存率的相关性。
Fas在浸润性导管癌中的表达低于导管内癌和正常组织。相反，NF-κB和VEGF-C在浸润性导管癌中的表达高于导管内癌和正常组织。Fas表达与肿瘤大小、组织学分级及临床病理分期相关；NF-κB表达与肿瘤大小、组织学分级、淋巴结转移相关；VEGF-C表达与乳腺癌的淋巴结转移及临床病理分期相关（p≤0.05）。Spearman等级相关分析显示，浸润性乳腺癌中Fas表达与NF-κB和VEGF-C表达均呈负相关（p<0.05）。此外，Kaplan-Meier生存分析表明，Fas阳性样本患者的5年生存率较高，而VEGF-C阳性样本患者的5年生存率较低。
本研究结果表明，Fas和NF-κB在乳腺癌的发生发展中起作用，而VEGF-C似乎促进淋巴结转移。因此，这些蛋白可能作为浸润性乳腺癌有用的诊断和预后标志物。
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