评估多形拟杆菌在土壤中存活的能力。
An evaluation of the ability of Dichelobacter nodosus to survive in soil.
机构信息
Department of Large Animal Science, Faculty of Health and Medical Sciences, University of Copenhagen, Grønnegårdsvej 3, DK-1870, Frederiksberg C, Denmark.
出版信息
Acta Vet Scand. 2013 Jan 23;55(1):4. doi: 10.1186/1751-0147-55-4.
BACKGROUND
Dichelobacter nodosus is the causative agent of footrot in sheep. The survival of the bacterium in soil is of importance for the epidemiology of the disease. The investigation evaluates the survival of D. nodosus in soil with and without added hoof powder stored under different temperatures.
RESULTS
An experimental setup was used with bacteriological culture and real-time polymerase chain reaction (PCR), and the results indicate that the bacteria can survive in soil for longer time than previously expected. The survival time was found to be dependent on temperature and the addition of hoof powder to the soil, with the longest survival time estimated to be 24 days in soil samples with hoof powder stored at 5°C.
CONCLUSION
Our findings indicate that the survival time of D. nodosus and its ability to infect susceptible sheep on pasture under different climatic conditions should be studied further.
背景
坏死梭杆菌是导致绵羊腐蹄病的病原体。该细菌在土壤中的存活对于疾病的流行病学具有重要意义。本研究评估了在不同温度下，有无添加蹄粉的情况下，土壤中坏死梭杆菌的存活情况。
结果
本研究采用细菌培养和实时聚合酶链反应（PCR）的实验设置，结果表明，与之前的预期相比，细菌在土壤中的存活时间更长。存活时间取决于温度以及向土壤中添加蹄粉，在 5°C 下储存添加蹄粉的土壤样本中，估计最长存活时间为 24 天。
结论
我们的研究结果表明，应进一步研究不同气候条件下，坏死梭杆菌的存活时间及其在牧场感染易感绵羊的能力。
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