UMR6197, Laboratoire de Microbiologie des Environnements Extrêmes, IUEM, Technopôle Brest-Iroise, F-29280 Plouzané, France.
Int J Syst Evol Microbiol. 2013 Aug;63(Pt 8):2920-2926. doi: 10.1099/ijs.0.026419-0. Epub 2013 Jan 25.
A novel hyperthermophilic, anaerobic archaeon, strain Bio-pl-0405IT2(T), was isolated from a hydrothermal chimney sample collected from the East Pacific Rise at 2700 m depth in the 'Sarah Spring' area (7° 25' 24" S 107° 47' 66" W). Cells were irregular, motile cocci (0.8-1.5 µm in diameter) and divided by constriction. Growth was observed at temperatures between 60 °C and 95 °C with an optimum at 80 °C. The pH range for growth was between pH 4.0 and pH 8.0 with an optimum around pH 7.0. Strain Bio-pl-0405IT2(T) grew at salt concentrations of 1-5 % (w/v) NaCl with an optimum at 2 %. The novel isolate grew by fermentation or sulphur respiration on a variety of organic compounds. It was a chemoorganoheterotrophic archaeon growing preferentially with yeast extract, peptone and tryptone as carbon and energy sources and sulphur and organic compounds as electron acceptors; it also grew on maltose and starch. Sulphur or l-cystine were required for growth and were reduced to hydrogen sulfide. The strain was resistant to rifampicin, chloramphenicol, vancomycin and kanamycin (all at 100 µg ml(-1)) but was sensitive to tetracycline. The G+C content of its genomic DNA was 53.6 mol%. Phylogenetic analysis of the almost complete 16S rRNA gene sequence (1450 bp) of strain Bio-pl-0405IT2(T) showed that the novel isolate belonged to the genus Thermococcus. DNA-DNA hybridization values with the two closest relatives Thermococcus hydrothermalis AL662(T) and Thermococcus celer JCM 8558(T) were below the threshold value of 70 %. On the basis of the physiological and genotypic distinctness, we propose a novel species, Thermococcus prieurii sp. nov. The type strain is Bio-pl-0405IT2(T) ( = CSUR P577(T)= JCM 16307(T)).
一种新型的嗜热、厌氧古菌，菌株 Bio-pl-0405IT2(T)，是从东太平洋海脊 2700 米深的“莎拉泉”（7°25'24"S 107°47'66"W）热液烟囱样本中分离出来的。细胞呈不规则、能动的球菌（直径 0.8-1.5μm），通过缢缩分裂。在 60-95°C 之间观察到生长，最佳温度为 80°C。生长的 pH 值范围在 4.0-8.0 之间，最佳 pH 值约为 7.0。菌株 Bio-pl-0405IT2(T) 在 1-5%（w/v）NaCl 的盐浓度下生长，最佳浓度为 2%。新型分离株通过发酵或硫呼吸在各种有机化合物上生长。它是一种化能有机异养古菌，优先以酵母提取物、蛋白胨和胰蛋白胨作为碳源和能源，以硫和有机化合物作为电子受体生长；它也可以在麦芽糖和淀粉上生长。生长需要硫或 L-胱氨酸，并将其还原为硫化氢。该菌株对利福平、氯霉素、万古霉素和卡那霉素（均为 100μg/ml）有抗性，但对四环素敏感。其基因组 DNA 的 G+C 含量为 53.6mol%。对菌株 Bio-pl-0405IT2(T) 的几乎完整的 16S rRNA 基因序列（1450bp）的系统发育分析表明，新型分离株属于 Thermococcus 属。与两个最接近的亲缘种 Thermococcus hydrothermalis AL662(T) 和 Thermococcus celer JCM 8558(T) 的 DNA-DNA 杂交值低于 70%的阈值。基于生理和基因型的独特性，我们提出了一个新种，Thermococcus prieurii sp. nov.。模式菌株是 Bio-pl-0405IT2(T)（=CSUR P577(T)=JCM 16307(T)）。
