College of Public Health, University of South China, Hengyang 421001, People's Republic of China.
Anal Biochem. 2013 May 1;436(1):45-52. doi: 10.1016/j.ab.2013.01.011. Epub 2013 Jan 26.
A simple and sensitive method for label-free, colorimetric detection of metallothioneins (MTs) has been developed by using a thymine (T)-rich oligonucleotide (TRO)-Hg-AuNP system. In this colorimetric strategy, the thiol groups of MTs could interact with mercury from the T-Hg(2+)-T complex to release TRO, resulting in a color change of the system. The response signals linearly correlated with the concentration of MTs over the range of 2.56 × 10(-8) to 3.08 × 10(-7) mol L(-1), and the limit of detection was 7.67 × 10(-9) mol L(-1). The relative standard deviation and the recovery were 2.3-4.8% (n = 11) and 94.2-103.9%, respectively. The proposed method avoids the label and derivatization steps in common methods, allows direct analysis of the samples by the naked eye without costly instruments, and is reliable, inexpensive, and sensitive.
一种简单灵敏的方法用于非标记、比色法检测金属硫蛋白（MTs）是通过使用胸腺嘧啶（T）丰富的寡核苷酸（TRO）-Hg-AuNP 系统。在这种比色策略中，MTs 的巯基可以与来自 T-Hg(2+)-T 配合物的汞相互作用，释放 TRO，导致系统颜色发生变化。该方法的响应信号与 MTs 的浓度在 2.56×10(-8)到 3.08×10(-7)mol L(-1)范围内呈线性相关，检测限为 7.67×10(-9)mol L(-1)。相对标准偏差和回收率分别为 2.3-4.8%（n=11）和 94.2-103.9%。该方法避免了常见方法中的标记和衍生化步骤，允许通过肉眼直接分析样品，而无需昂贵的仪器，并且可靠、廉价、灵敏。
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