PAF拮抗剂WEB-2086、L-652,731和SRI-63441对中性粒细胞和嗜酸性粒细胞对PAF趋化反应的抑制作用。

Inhibition of neutrophil and eosinophil chemotactic responses to PAF by the PAF-antagonists WEB-2086, L-652,731, and SRI-63441.

作者信息

Håkansson L, Venge P

机构信息

Department of Clinical Chemistry, University Hospital, Uppsala, Sweden.

出版信息

J Leukoc Biol. 1990 May;47(5):449-56. doi: 10.1002/jlb.47.5.449.

DOI:10.1002/jlb.47.5.449

PMID:2335754

Abstract

The influence of the three PAF-antagonists WEB-2086, L-652,731, and SRI-63441 on the chemotactic response of neutrophil and eosinophil granulocytes to PAF was investigated. When the PAF-antagonists were added to the cell suspension that was exposed to a gradient of PAF, WEB-2086 and SRI-63441 at the concentration of 10(-6) mol/litre inhibited (P less than .01) the neutrophil and eosinophil chemotactic response to 10(-8) and 10(-9) mol PAF per litre; at the concentration of 5 x 10(-6) mol/litre, WEB-2086 and SRI-63441 also inhibited (P less than .02) the response to 10(-7) mol PAF per litre. Under the same conditions L-652,731 at the concentration of 5 x 10(-6) mol/litre inhibited (P less than .01) the eosinophil chemotactic response to 10(-8) and 10(-9) mol PAF per litre. The inhibition of the chemotactic response to PAF by the three PAF-antagonists was specific, since the chemotactic response to C5f, f-MLP, and LTB4 was not affected by WEB-2086, L-652,731, or SRI-63441, neither was the chemokinetic migration induced by albumin.

摘要

研究了三种血小板活化因子（PAF）拮抗剂WEB-2086、L-652,731和SRI-63441对中性粒细胞和嗜酸性粒细胞向PAF趋化反应的影响。当将PAF拮抗剂添加到暴露于PAF梯度的细胞悬液中时，浓度为10^(-6)摩尔/升的WEB-2086和SRI-63441抑制（P<0.01）了中性粒细胞和嗜酸性粒细胞对每升10^(-8)和10^(-9)摩尔PAF的趋化反应；在浓度为5×10^(-6)摩尔/升时，WEB-2086和SRI-63441也抑制（P<0.02）了对每升10^(-7)摩尔PAF的反应。在相同条件下，浓度为5×10^(-6)摩尔/升的L-652,731抑制（P<0.01）了嗜酸性粒细胞对每升10^(-8)和10^(-9)摩尔PAF的趋化反应。这三种PAF拮抗剂对PAF趋化反应的抑制是特异性的，因为对C5f、f-MLP和LTB4的趋化反应不受WEB-2086、L-652,731或SRI-63441的影响，白蛋白诱导的化学促动性迁移也不受影响。