利用固定化在石墨烯上的胰蛋白酶实现高效的组织内原位酶切，用于 MALDI 成像分析的组织蛋白鉴定。
Realization of on-tissue protein identification by highly efficient in situ digestion with graphene-immobilized trypsin for MALDI imaging analysis.
机构信息
Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, China.
出版信息
Analyst. 2013 Mar 21;138(6):1645-8. doi: 10.1039/c3an36391k. Epub 2013 Jan 31.
PMID:23364134
Abstract
A novel implementation of in situ protein digestion supported by a graphene oxide-immobilized enzyme reactor (GO-IMER) in the MALDI imaging mass spectrometry (IMS) workflow is reported, which enables the simultaneous diagnostic identity and distribution attributes of the proteome on tissue.
摘要
报道了一种新型的原位蛋白质消化方法，该方法通过石墨烯氧化物固定化酶反应器（GO-IMER）在 MALDI 成像质谱（IMS）工作流程中实现，可同时对组织中的蛋白质组进行诊断鉴定和分布属性分析。
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