[BTW5对日本血吸虫成虫和幼虫的体外杀血吸虫作用]
[Schistosomicidal effect of BTW5 on adult and juvenile Schistosoma japonicum in vitro].
作者信息
Liu Chen-Chen, Zhang Jing, Gao Si-Jia, Zhou Xia, Gong Wei, Yang Shi-Lin, Xu Qiong-Ming, Zhu-Ge Hong-Xiang
机构信息
Department of Pathogenic Biology, School of Basic Medicine and Bioscience, Medical College of Soochow University, Suzhou 215123, China.
出版信息
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2012 Oct;24(5):533-6.
OBJECTIVE
To evaluate the killing effect of BTW5 on both juvenile and adult worms of Schistosoma japonicum in vitro.
METHODS
The mice were infected with cercariae of Schistosoma japonicum obtained from infected Oncomelania hupensis. The juvenile worms were obtained from the liver and mesenteric veins of the mice by perfusion 18 d after the infection, and the adult worms were obtained from the liver and mesenteric veins of the mice by perfusion 5 weeks after the infection. The adult and juvenile worms were cultivated in DMEM medium containing different concentrations of BTW5 and were observed for 3 d. After the observation, the worms were stained by hydrochloric acid carmine to observe the injuries.
RESULTS
The death rates and motility reducing rates of adult and juvenile worms in DMEM medium containing different concentrations of BTW5 were significantly higher than those in the controls. The staining suggested that BTW5 had a damage effect on the tegument and the intestinal canal of the male and female worms, in addition to the ovaries of the female worms.
CONCLUSION
BTW5 has an antischistosomal effect on adult and juvenile worms of Oncomelania hupensis.
目的
评估BTW5对日本血吸虫成虫和幼虫的体外杀灭作用。
方法
用从感染的湖北钉螺获取的日本血吸虫尾蚴感染小鼠。感染18天后通过灌注从小鼠肝脏和肠系膜静脉获取幼虫，感染5周后通过灌注从小鼠肝脏和肠系膜静脉获取成虫。将成虫和幼虫培养于含有不同浓度BTW5的DMEM培养基中，观察3天。观察后，用盐酸卡红对虫体进行染色以观察损伤情况。
结果
含有不同浓度BTW5的DMEM培养基中，成虫和幼虫的死亡率及活动率降低率均显著高于对照组。染色显示，BTW5除对雌虫卵巢有损伤作用外，对雌雄虫的体表和肠道均有损伤作用。
结论
BTW5对日本血吸虫成虫和幼虫均有抗血吸虫作用。
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