Department of Biochemistry, University of Allahabad, Allahabad, India.
Arch Med Res. 2013 Feb;44(2):136-41. doi: 10.1016/j.arcmed.2013.01.003. Epub 2013 Jan 30.
Oxidative stress is thought to play a major role in the development of several age-dependent diseases. Proteins are major targets for oxidative attack. Protein hydroperoxides are formed by hydroxyl and singlet oxygen attack on protein, forming relatively stable hydroperoxides on histidine, tyrosine and tryptophan residues. This study investigated the levels of plasma protein hydroperoxides and antioxidant potential of plasma during aging in humans. We correlated the protein hydroperoxide formation with plasma antioxidant potential, paraoxonase 1 (PON1) arylesterase activity and plasma total thiols.
The protein hydroperoxides and antioxidant potential were measured in plasma of human subjects aged between 20 and 81 years of both genders.
Increase in plasma protein hydroperoxides and decrease in plasma antioxidant potential were observed as function of human age.
This study provides strong correlation between plasma protein hydroperoxides formation and decrease in plasma antioxidant potential during aging. PON1 arylesterase activity and plasma total thiols levels were also found to show significant correlation with increasing levels of plasma protein hydroperoxides during aging. The plasma protein hydroperoxides provide a reliable marker of long-term redox balance and degree of oxidative stress during aging process.
氧化应激被认为在几种与年龄相关的疾病的发展中起主要作用。蛋白质是氧化攻击的主要靶标。蛋白质过氧化物是由羟基和单线态氧对蛋白质的攻击形成的，在组氨酸、酪氨酸和色氨酸残基上形成相对稳定的过氧化物。本研究调查了人类衰老过程中血浆蛋白过氧化物水平和血浆抗氧化潜能。我们将蛋白过氧化物的形成与血浆抗氧化潜能、对氧磷酶 1（PON1）芳基酯酶活性和血浆总巯基相关联。
测量了 20 至 81 岁的男性和女性受试者血浆中的蛋白过氧化物和抗氧化潜能。
随着人类年龄的增长，观察到血浆蛋白过氧化物的增加和血浆抗氧化潜能的降低。
本研究提供了在衰老过程中血浆蛋白过氧化物形成与血浆抗氧化潜能降低之间的强相关性。PON1 芳基酯酶活性和血浆总巯基水平也被发现与衰老过程中血浆蛋白过氧化物水平的升高呈显著相关。血浆蛋白过氧化物为长期氧化还原平衡和氧化应激程度提供了可靠的标志物。
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