Konishi T
Department of Neurology, Utano National Hospital, Kyoto, Japan.
Brain Res. 1990 Jan 29;508(1):85-92. doi: 10.1016/0006-8993(90)91121-v.
Dye coupling and voltage-dependent ionic currents were evaluated simultaneously in mouse Schwann cells using patch pipettes containing Lucifer yellow. Dye coupling was examined in different types of cultured Schwann cells: cells not associated with axons, non-myelinating cells associated with axons, and myelinating cells. Myelinating and non-myelinating cells were also examined in freshly dissociated adult nerves. The incidence of dye coupling was higher in embryonic dorsal root ganglia (DRG) than in neonatal sciatic nerves. In both types of cultures the incidence of dye coupling decreased with time. In DRG cultures voltage-dependent K+ currents were observed in the Schwann cells, except in some of the myelinating cells, suggesting that K+ currents disappear as myelin formation progresses, and dye coupling was eliminated in myelinating cells. In freshly dissociated adult nerves, large myelinating Schwann cells lacked both K+ currents and dye coupling which were preserved in non-myelinating cells. Although the association between dye coupling and voltage-dependent K+ channels in non-myelinating cells is not clear, Schwann cells which had differentiated to engage in myelin formation lost both properties.
使用含有荧光黄的膜片吸管在小鼠雪旺细胞中同时评估染料偶联和电压依赖性离子电流。在不同类型的培养雪旺细胞中检测染料偶联：未与轴突相关的细胞、与轴突相关的无髓鞘细胞和有髓鞘细胞。还在新鲜分离的成年神经中检查了有髓鞘细胞和无髓鞘细胞。胚胎背根神经节（DRG）中染料偶联的发生率高于新生坐骨神经。在两种培养类型中，染料偶联的发生率均随时间下降。在DRG培养物中，除了一些有髓鞘细胞外，在雪旺细胞中观察到电压依赖性钾电流，这表明随着髓鞘形成的进展钾电流消失，并且在有髓鞘细胞中染料偶联被消除。在新鲜分离的成年神经中，大型有髓鞘雪旺细胞既缺乏钾电流也缺乏染料偶联，而在无髓鞘细胞中这些特性得以保留。尽管无髓鞘细胞中染料偶联与电压依赖性钾通道之间的关联尚不清楚，但已分化参与髓鞘形成的雪旺细胞失去了这两种特性。
