端粒缩短可区分倒置性尿路上皮肿瘤。

Telomere shortening distinguishes inverted urothelial neoplasms.

机构信息

Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN 46202, USA.

出版信息

Histopathology. 2013 Mar;62(4):595-601. doi: 10.1111/his.12030. Epub 2013 Feb 5.

DOI:10.1111/his.12030

PMID:23379729

Abstract

AIMS

To investigate relative telomere length in inverted urothelial neoplasms, including inverted papilloma and urothelial carcinoma with an inverted growth pattern. Telomere shortening has been implicated as an early event in the development of epithelial malignancies in a number of organ systems.

METHODS AND RESULTS

Formalin-fixed, paraffin-embedded tissue sections from 77 cases were studied, including 26 cases of inverted papilloma, 26 urothelial carcinomas with inverted growth, and 25 cases of cystitis glandularis. Quantitative fluorescence in-situ hybridization (FISH) was performed on interphase nuclei, utilizing a telomere-specific peptide nucleic acid probe to assess telomeric signal intensity. Relative telomere lengths for urothelial carcinoma with inverted growth, cystitis glandularis and inverted papilloma were 29%, 84%, and 91%, respectively. A statistically significant reduction in relative telomere length was present between urothelial carcinoma with inverted growth and inverted papilloma (P < 0.001); no significant difference was detected between normal urothelium, cystitis glandularis, and inverted papilloma.

CONCLUSIONS

Significant telomere shortening in urothelial carcinoma with inverted growth as compared with inverted papilloma distinguishes the two lesions, and supports the notion that inverted papilloma is a benign neoplasm with a distinct pathogenetic mechanism of development. Telomeric FISH analysis may be a useful biomarker in distinguishing inverted papilloma from urothelial carcinoma with inverted growth.

摘要

目的

研究倒置性尿路上皮肿瘤（包括 inverted papilloma 和 inverted growth pattern 的 urothelial carcinoma）中的相对端粒长度。端粒缩短已被认为是许多器官系统中上皮恶性肿瘤发展的早期事件。

方法和结果

对 77 例福尔马林固定、石蜡包埋的组织切片进行了研究，包括 26 例 inverted papilloma、26 例 inverted growth 的 urothelial carcinomas 和 25 例腺性膀胱炎。利用端粒特异性肽核酸探针进行间期核定量荧光原位杂交（FISH），评估端粒信号强度。inverted growth 的 urothelial carcinomas、cystitis glandularis 和 inverted papilloma 的相对端粒长度分别为 29%、84%和 91%。inverted growth 的 urothelial carcinomas 与 inverted papilloma 之间的相对端粒长度显著降低（P < 0.001）；正常尿路上皮、cystitis glandularis 和 inverted papilloma 之间无显著差异。

结论

与 inverted papilloma 相比，inverted growth 的 urothelial carcinomas 中存在显著的端粒缩短，这区分了两种病变，并支持 inverted papilloma 是一种良性肿瘤，其发病机制明显不同的观点。端粒 FISH 分析可能是区分 inverted papilloma 和 inverted growth 的 urothelial carcinomas 的有用生物标志物。