Nguyen Linh Viet, Warren-Smith Stephen C, Cooper Alan, Monro Tanya M
Institute for Photonics & Advanced Sensing, The University of Adelaide, Adelaide SA 5005, Australia.
Opt Express. 2012 Dec 31;20(28):29378-85. doi: 10.1364/OE.20.029378.
We propose and experimentally demonstrate a new class of sensor for specific DNA sequences based on molecular beacons (MB) immobilized on the internal surfaces of suspended core optical fibers (SCF). MBs, a type of hairpin structured DNA probe, are attached on the surface of the SCF core using a fuzzy nanoassembly process used in conjunction with a biotin-streptavidin-biotin surface attachment strategy. The proposed DNA sensor detects complementary DNA sequences (cDNA) while discriminating sequences differing from the target by just one base. This enables the detection of DNA in unprecedentedly small sample volumes (nL scale) and is, to the best of our knowledge, the first specific DNA detection using a DNA probe immobilized within a microstructured optical fiber.
我们提出并通过实验证明了一种基于固定在悬浮芯光纤（SCF）内表面的分子信标（MB）的新型特定DNA序列传感器。MB是一种发夹结构的DNA探针，通过与生物素-链霉亲和素-生物素表面附着策略结合使用的模糊纳米组装过程附着在SCF芯表面。所提出的DNA传感器可检测互补DNA序列（cDNA），同时区分与目标序列仅相差一个碱基的序列。这使得能够在前所未有的小样本体积（纳升规模）中检测DNA，据我们所知，这是首次使用固定在微结构光纤内的DNA探针进行特定DNA检测。
