Department of Chemistry, Fudan University, 220 Handan Road, Shanghai, 200433, China.
Nanoscale Res Lett. 2013 Feb 7;8(1):63. doi: 10.1186/1556-276X-8-63.
Self-assembled monolayers (SAMs) of pyridylthio-functionalized multiwalled carbon nanotubes (pythio-MWNTs) have been constructed on the gold substrate surface, which were used as a support to immobilize cytochrome c (Cyt c). The assembly processes of the SAMs and adsorption of Cyt c were monitored by using quartz crystal microbalance (QCM). Based on the frequency change of the QCM resonator, the surface coverage for the SAMs of pythio-MWNTs was estimated to be about 5.2 μg/cm2, and that of the Cyt c adsorbed was about 0.29 μg/cm2. For the gold electrode modified by the SAMs of pythio-MWNTs-Cyt c, a quasi-reversible redox wave was recorded with the cathodic and anodic potentials at about -0.55 and -0.28 V vs Ag/AgCl, respectively. Compositions and morphologies of the SAMs before and after immobilization of Cyt c were characterized by X-ray photoelectron spectroscopy, Raman spectroscopy, scanning electron microscopy, and atomic force microscopy.
已在金基底表面构建了吡啶硫基功能化多壁碳纳米管（pythio-MWNTs）的自组装单分子层（SAMs），并将其用作固定细胞色素 c（Cyt c）的支撑物。通过使用石英晶体微天平（QCM）监测 SAMs 的组装过程和 Cyt c 的吸附。基于 QCM 谐振器的频率变化，估计 pythio-MWNTs 的 SAM 的表面覆盖率约为 5.2μg/cm2，吸附的 Cyt c 的表面覆盖率约为 0.29μg/cm2。对于由 pythio-MWNTs-Cyt c SAM 修饰的金电极，记录到准可逆氧化还原波，其阴极和阳极电位分别约为-0.55 和-0.28 V 相对于 Ag/AgCl。通过 X 射线光电子能谱、拉曼光谱、扫描电子显微镜和原子力显微镜对 Cyt c 固定前后的 SAM 的组成和形态进行了表征。
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