Buscail L, Cauvin A, Gourlet P, Gossen D, De Neef P, Rathé J, Robberecht P, Vandermeers-Piret M C, Vandermeers A, Christophe J
Department of Biochemistry and Nutrition, Medical School, Université Libre de Bruxelles, Belgium.
Biochim Biophys Acta. 1990 May 8;1038(3):355-9. doi: 10.1016/0167-4838(90)90248-e.
The neuropeptides vasoactive intestinal peptide (VIP) and peptide histidine isoleucinamide (1-27) (PHI) and the hormone secretin were purified from the small intestine of guinea pig, being detected by radioimmunoassay and radioreceptor assay throughout six to seven chromatographic steps. After elution on a reverse-phase C18 column, the three peptides were separated on a Fractogel column. After cation-exchange chromatography of each peptide on Mono S, the final steps were performed using a reverse-phase RP8-e column. Guinea pig PHI differed from porcine PHI in having Tyr and Arg residues instead of Phe and Lys in, respectively, position 10 and 20. We confirmed the original sequence of guinea pig VIP previously documented (with Leu5, Thr9, Met19 and Val26). We also established the similarity of the primary structure of guinea pig secretin with that of porcine and bovine.
从豚鼠小肠中纯化出神经肽血管活性肠肽（VIP）、肽组氨酸异亮氨酸酰胺（1 - 27）（PHI）和激素促胰液素，通过放射免疫分析和放射受体分析在六到七个色谱步骤中对其进行检测。在反相C18柱上洗脱后，这三种肽在Fractogel柱上分离。每种肽在Mono S上进行阳离子交换色谱后，最后步骤使用反相RP8 - e柱进行。豚鼠PHI与猪PHI的不同之处在于，在第10位和第20位分别具有酪氨酸和精氨酸残基，而不是苯丙氨酸和赖氨酸。我们证实了先前记录的豚鼠VIP的原始序列（Leu5、Thr9、Met19和Val26）。我们还确定了豚鼠促胰液素的一级结构与猪和牛的促胰液素的一级结构相似。
