文献检索，用中文搜 PubMed

Suppr 超能文献

核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Ohguro H, Fukada Y, Saito T, Akino T

Department of Biochemistry, Sapporo Medical College, Japan.

Comp Biochem Physiol B. 1990;95(4):763-5. doi: 10.1016/0305-0491(90)90314-j.

Transducin subunits (T alpha and T beta gamma) were purified from freshly dissected frog (Rana catesbeiana) retinas. It was found that purified T beta gamma is composed of three components which can be separated from each other by an anion exchange column chromatography under nondenaturing conditions. 2. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analyses of these three components demonstrated that each contains T beta (mol. wt 35,000) and T gamma (mol. wt approximately 8000). 3. Only one of the three components retained an ability to enhance the binding of GppNHp to T alpha in the presence of a photobleaching intermediate of rhodopsin, while the others showed very low abilities to enhance the binding. 4. These observations, together with the similar findings on bovine T beta gamma, strongly suggest that the functional heterogeneity of T beta gamma is conserved in vertebrate photoreceptor cells.

转导蛋白亚基（Tα和Tβγ）从刚解剖的牛蛙（Rana catesbeiana）视网膜中纯化得到。研究发现，纯化后的Tβγ由三个组分组成，在非变性条件下可通过阴离子交换柱色谱将它们彼此分离。2. 对这三个组分进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析表明，每个组分都含有Tβ（分子量35,000）和Tγ（分子量约8000）。3. 在视紫红质的光漂白中间体存在的情况下，三个组分中只有一个保留了增强GppNHp与Tα结合的能力，而其他组分增强结合的能力非常低。4. 这些观察结果，连同对牛Tβγ的类似发现，有力地表明Tβγ的功能异质性在脊椎动物光感受器细胞中是保守的。

Ohguro H, Fukada Y, Saito T, Akino T

Department of Biochemistry, Sapporo Medical College, Japan.

Comp Biochem Physiol B. 1990;95(4):763-5. doi: 10.1016/0305-0491(90)90314-j.

Transducin subunits (T alpha and T beta gamma) were purified from freshly dissected frog (Rana catesbeiana) retinas. It was found that purified T beta gamma is composed of three components which can be separated from each other by an anion exchange column chromatography under nondenaturing conditions. 2. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analyses of these three components demonstrated that each contains T beta (mol. wt 35,000) and T gamma (mol. wt approximately 8000). 3. Only one of the three components retained an ability to enhance the binding of GppNHp to T alpha in the presence of a photobleaching intermediate of rhodopsin, while the others showed very low abilities to enhance the binding. 4. These observations, together with the similar findings on bovine T beta gamma, strongly suggest that the functional heterogeneity of T beta gamma is conserved in vertebrate photoreceptor cells.

转导蛋白亚基（Tα和Tβγ）从刚解剖的牛蛙（Rana catesbeiana）视网膜中纯化得到。研究发现，纯化后的Tβγ由三个组分组成，在非变性条件下可通过阴离子交换柱色谱将它们彼此分离。2. 对这三个组分进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析表明，每个组分都含有Tβ（分子量35,000）和Tγ（分子量约8000）。3. 在视紫红质的光漂白中间体存在的情况下，三个组分中只有一个保留了增强GppNHp与Tα结合的能力，而其他组分增强结合的能力非常低。4. 这些观察结果，连同对牛Tβγ的类似发现，有力地表明Tβγ的功能异质性在脊椎动物光感受器细胞中是保守的。