Sharma Sonia, Gupta Anshu, Kalhan Shivani, Dudani Sharmila, Sharma Pankaj, Devra Amit
Assisstant Professor, Department of Pathology, Army College of Medical Sciences , Delhi Cantt, India .
J Clin Diagn Res. 2013 Jan;7(1):79-81. doi: 10.7860/JCDR/2012/4964.2675. Epub 2013 Jan 1.
Renal transplantation is the definitive treatment in renal failure patients. Liver disease is a known problem in renal transplant recipients. They may be consequent to immunosuppression, drug toxicity, altered immune response to viruses and hemodialysis.
The aim of this study was to analyze and correlate the biochemical parameters and histopathology of liver biopsy among renal transplant recipients with both HBV and HCV infection and to correlate them.
The study group had thirty cases. Enrolment criteria included coinfection with HBV and HCV ; elevated liver enzymes and recipient of renal allograft. There was acontrol group of ten patients who were HBC and HCV positive but had not undergone renal transplant.
MATERIAL & METHODS: Liver function tests including alkaline phosphatase, SGOT, SGPT and serum bilirubin levels were donet. Percutaneous liver biopsies were carried out using Menghini's needle.. Stains done included hematoxylin and eosin (H & E), vanGieson, reticulin and Perl's stain. Histopathological grading was performed using Metavir scoring system. Immunohistochemistry (IHC) was done where required for ground glass hepatocytes. Correlation of SGOT, SGPT and Alkaline phosphatase of the study group and the controls was carried out with the grading.
Statistical tests done included paired "t" test at 5% and test of probability.
There was no statistically significant correlation between the controls and the transplanted patients. It was concluded that serum enzyme levels could be used to predict histological grade in the control group but not in the transplant recipients (p>0.05).
肾移植是肾衰竭患者的最终治疗方法。肝脏疾病是肾移植受者中已知的问题。它们可能是免疫抑制、药物毒性、对病毒的免疫反应改变以及血液透析的结果。
本研究的目的是分析和关联同时感染乙肝病毒（HBV）和丙肝病毒（HCV）的肾移植受者肝脏活检的生化参数和组织病理学，并将它们进行关联。
研究组有30例病例。纳入标准包括HBV和HCV合并感染；肝酶升高以及肾移植受者。有一个对照组，由10名HBc和HCV阳性但未接受肾移植的患者组成。
进行肝功能检查，包括碱性磷酸酶、谷草转氨酶（SGOT）、谷丙转氨酶（SGPT）和血清胆红素水平检测。使用Menghini针进行经皮肝活检。所做的染色包括苏木精和伊红（H&E）染色、范吉森染色、网状纤维染色和Perl染色。使用梅塔维尔评分系统进行组织病理学分级。必要时对毛玻璃样肝细胞进行免疫组织化学（IHC）检测。对研究组和对照组的SGOT、SGPT和碱性磷酸酶与分级进行相关性分析。
所做的统计检验包括5%的配对“t”检验和概率检验。
对照组与移植患者之间无统计学显著相关性。得出的结论是，血清酶水平可用于预测对照组的组织学分级，但不能用于预测移植受者的组织学分级（p>0.05）。
