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来自3个潜在来源(骨髓、骨膜和脂肪组织)的大鼠间充质干细胞的生长动力学。

Growth kinetics of rat mesenchymal stem cells from 3 potential sources: bone marrow, periosteum and adipose tissue.

作者信息

Tawonsawatruk Tulyapruek, Spadaccino Antonio, Murray Iain R, Peault Bruno, Simpson Hamish A H R W S

机构信息

Department of Orthopaedic, Royal Infirmary of Edinburgh Hospital, The University of Edinburgh, Scotland.

出版信息

J Med Assoc Thai. 2012 Oct;95 Suppl 10:S189-97.

Abstract

OBJECTIVE

Mesenchymal stem cells (MSCs) have potential in orthopaedic applications as they are able to differentiate into bone and cartilage. These cells can be isolated from a variety of adult tissues. Three sources that are relevant for orthopaedic applications are bone marrow, periosteum and adipose tissue. The purpose of the present study was to compare the growth kinetics and colony forming potency of rat MSCs from these sources.

MATERIAL AND METHOD

Bone marrow from the femur periosteum from the femoral diaphysis and adipose tissue from the inguinal area of Wistar rats were harvested for MSC isolation. The cells from 2nd-4th passage from primary culture were selected for study of their growth curves, population doubling time and colony forming ability using the percentage of colony forming units and colony forming area as the outcome measure.

RESULTS

The isolated cells from these 3 sources were capable of osteogenesis, chondrogenesis and adipogenesis. The growth kinetics were compared using the growth curve and the population doubling time (PDT): bone marrow derived cells (PDT = 3.99 days, SD = 1.19) and periosteum derived cells (PDT = 3.55 days, SD = 1.21) had faster growth kinetics than adipose derived cells (PDT = 4.65 days, SD = 1.53). The percentage of colony forming units and the colony forming area from bone marrow derived cells (% colony forming unit = 8.58, SD = 1.35 and % colony forming area = 25.12, SD = 7.31) and periosteum derived cells (% colony forming units = 9.92, SD = 2.06, % colony forming area = 32.45, SD = 10.74) were significantly greater (p < 0. 05) than adipose derived cells (% colony forming units = 5.92, SD = 0.78, % colony forming area = 15.80, SD = 9.035).

CONCLUSION

The growth kinetics and colony forming potency of MSCs from bone marrow and periosteum were comparable. The bone marrow and periosteum should be a suitable source for MSC isolation. The growth kinetics of MSCs derived from adipose tissue was lower than the other sources. Adipose tissue can be used as an alternative source as it is readily available and dispensable.

摘要

目的

间充质干细胞(MSCs)具有分化为骨和软骨的能力,在骨科应用中具有潜力。这些细胞可从多种成人组织中分离得到。与骨科应用相关的三个来源是骨髓、骨膜和脂肪组织。本研究的目的是比较来自这些来源的大鼠MSCs的生长动力学和集落形成能力。

材料与方法

采集Wistar大鼠股骨骨髓、股骨干骨膜和腹股沟区脂肪组织用于分离MSCs。选择原代培养第2 - 4代的细胞,以集落形成单位百分比和集落形成面积作为观察指标,研究其生长曲线、群体倍增时间和集落形成能力。

结果

从这3个来源分离的细胞均具有成骨、成软骨和成脂能力。通过生长曲线和群体倍增时间(PDT)比较生长动力学:骨髓来源的细胞(PDT = 3.99天,标准差 = 1.19)和骨膜来源的细胞(PDT = 3.55天,标准差 = 1.21)的生长动力学比脂肪来源的细胞(PDT = 4.65天,标准差 = 1.53)更快。骨髓来源的细胞(集落形成单位百分比 = 8.58,标准差 = 1.35;集落形成面积百分比 = 25.12,标准差 = 7.31)和骨膜来源的细胞(集落形成单位百分比 = 9.92,标准差 = 2.06;集落形成面积百分比 = 32.45,标准差 = 10.74)的集落形成单位百分比和集落形成面积显著大于(p < 0.05)脂肪来源的细胞(集落形成单位百分比 = 5.92,标准差 = 0.78;集落形成面积百分比 = 15.80,标准差 = 9.035)。

结论

骨髓和骨膜来源的MSCs的生长动力学和集落形成能力相当。骨髓和骨膜应是分离MSCs的合适来源。脂肪组织来源的MSCs的生长动力学低于其他来源。脂肪组织因其容易获取且可舍弃,可作为替代来源。

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