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一株韩国本土微藻 Chlamydomonas reinhardtii KNUA021 的分离及其生物技术潜力分析。

Isolation of a Korean domestic microalga, Chlamydomonas reinhardtii KNUA021, and analysis of its biotechnological potential.

机构信息

Department of Biology, Kyungpook National University, Daegu 702-701, Korea, Advanced Bio-resource Research Center, Kyungpook National University, Daegu 702-701, Korea.

出版信息

J Microbiol Biotechnol. 2013 Mar;23(3):375-81. doi: 10.4014/jmb.1111.11073.

DOI:10.4014/jmb.1111.11073
PMID:23462011
Abstract

A freshwater microalga, Chlamydomonas reinhardtii KNUA021, was characterized for its potential as a biochemical feedstock. Its optimal growth was observed when the culture was incubated at 25°C and pH 9.4. However, the isolate was capable of survival and growth under a variety of temperatures (10-30°C) and pH (pH 4.0-12.0) conditions. The total lipid content of the isolate was 21.7% of dry weight and it was found that a high-value fatty alcohol, hexadecenol (C(20)H(40)O), was autotrophically produced by strain KNUA021. In addition, a nutritionally important C(18:3) ω3 (α-linolenic acid, ALA) was also identified in this photosynthetic microorganism as one of the major fatty acids. Hence, C. reinhardtii KNUA021 appears to show promise for use in the production of microalgae-based biochemicals.

摘要

一株淡水微藻,莱茵衣藻 KNUA021,因其作为生物化学原料的潜力而被描述。当培养温度为 25°C 且 pH 值为 9.4 时,该藻种的生长最佳。然而,该分离株能够在各种温度(10-30°C)和 pH 值(pH 值 4.0-12.0)条件下存活和生长。分离株的总脂含量为干重的 21.7%,发现一株高产值脂肪醇,十六烯醇(C(20)H(40)O),由 KNUA021 菌株通过自养方式生产。此外,在这种光合微生物中还发现了一种重要的营养物质 C(18:3) ω3(α-亚麻酸,ALA),它是主要脂肪酸之一。因此,莱茵衣藻 KNUA021 似乎有望用于生产基于微藻的生物化学物质。

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