College of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou, China.
Electrophoresis. 2013 May;34(9-10):1383-9. doi: 10.1002/elps.201200589. Epub 2013 Apr 16.
This is the first time when protein-imprinted polymers are prepared with "pending templates." The polymers were synthesized in the presence of a real sample (chicken egg white), rather than any known commercial proteins. Compared with a simultaneously synthesized nonimprinted control polymer, the polymers show higher adsorption capacity for abundant components (as "pending templates") in the original sample. Chromatography experiments indicated that the columns made of the imprinted polymers could retain abundant species (imprinted) and separate them from those not imprinted. Thus, the sample could be split into dimidiate subfractions with reduced complexities. "Pending template imprinting" suggests a new way to investigate molecular imprinting, especially to dissect, simplify, and analyze complicated samples through a series of polymers just imprinted by the samples per se.
这是首次使用“潜在模板”制备蛋白质印迹聚合物。该聚合物是在真实样品（鸡蛋白）存在的情况下合成的，而不是任何已知的商业蛋白质。与同时合成的非印迹对照聚合物相比，聚合物对原始样品中丰富的成分（作为“潜在模板”）表现出更高的吸附能力。色谱实验表明，由印迹聚合物制成的色谱柱可以保留丰富的物质（印迹）并将其与未印迹的物质分离。因此，样品可以被分成复杂度降低的两个亚部分。“潜在模板印迹”为分子印迹的研究提供了一种新方法，特别是通过一系列仅由样品本身印迹的聚合物来剖析、简化和分析复杂的样品。
