Laboratory for Analytical Chemistry, Croatian Veterinary Institute, 10000 Zagreb, Croatia.
J Anal Toxicol. 2013 May;37(4):241-5. doi: 10.1093/jat/bkt017. Epub 2013 Mar 12.
The aim of the study was to evaluate the adequacy of enzyme-linked immunosorbent assay (ELISA) in the post-exposure determination of the β-agonists clenbuterol and salbutamol in animal plasma and serum. Experimental guinea pigs (n = 20) were treated with two doses (0.25 and 2.5 mg/kg) of clenbuterol (n = 10) and salbutamol (n = 10) for seven days, whereas the control animal group (n = 10) was left untreated. Validation of the applied method yielded acceptable recovery (mean > 70%) and repeatability rates, showing ELISA to be applicable for the semi-quantitative determination of both analytes in both matrices, preferably in plasma. In both matrices, clenbuterol concentrations were proven to be significantly (14-fold) higher than those of salbutamol. Concentrations of both analytes were higher in plasma than in serum. The application of a 10-fold higher clenbuterol and salbutamol dose (2.5 mg/kg) resulted in concentrations 3- to 4-fold higher for clenbuterol and 2- to 3-fold higher for salbutamol, indicating a different release rate of these two β-agonists.
本研究旨在评估酶联免疫吸附测定（ELISA）在动物血浆和血清中β-激动剂克仑特罗和沙丁胺醇暴露后检测中的适用性。实验豚鼠（n=20）接受了两种剂量（0.25 和 2.5 mg/kg）的克仑特罗（n=10）和沙丁胺醇（n=10）治疗，为期 7 天，而对照组动物（n=10）则未接受治疗。所应用方法的验证结果表明，回收率（平均 >70%）和重复性良好，表明 ELISA 适用于两种分析物在两种基质中的半定量测定，优选在血浆中。在两种基质中，克仑特罗的浓度均明显（14 倍）高于沙丁胺醇。两种分析物在血浆中的浓度均高于血清。应用 10 倍更高剂量的克仑特罗（2.5 mg/kg）和沙丁胺醇（2.5 mg/kg），导致克仑特罗的浓度升高 3-4 倍，沙丁胺醇的浓度升高 2-3 倍，表明这两种β-激动剂的释放率不同。
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