转互补系统是否适合丙型肝炎病毒生命周期研究？
Are trans-complementation systems suitable for hepatitis C virus life cycle studies?
机构信息
EA4294 Unité de Virologie Clinique et Fondamentale, Université de Picardie Jules Verne, Amiens, France.
出版信息
J Viral Hepat. 2013 Apr;20(4):225-33. doi: 10.1111/jvh.12069. Epub 2013 Feb 14.
Abstract
Complementation is a naturally occurring genetic mechanism that has been studied for a number of plus-strand RNA viruses. Although trans-complementation is well documented for Flaviviridae family viruses, the first such system for hepatitis C virus (HCV) was only described in 2005. Since then, the development of a number of HCV trans-complementation models has improved our knowledge of HCV protein functions and interactions, genome replication and viral particle assembly. These models have also been used to produce defective viruses and so improvements are necessary for vaccine assays. This review provides an update on HCV trans-complementation systems, the viral mechanisms studied therewith and the production and characterization of trans-encapsidated particles.
摘要
互补作用是一种自然发生的遗传机制，已经有许多正链 RNA 病毒对此进行了研究。尽管 Flaviviridae 科的病毒的转互补作用已有充分的文献记载，但直到 2005 年才首次描述了丙型肝炎病毒（HCV）的这种系统。此后，许多 HCV 转互补模型的发展提高了我们对 HCV 蛋白功能和相互作用、基因组复制和病毒颗粒组装的认识。这些模型也被用于产生缺陷病毒，因此疫苗检测需要进行改进。本文综述了 HCV 转互补系统、与之相关的病毒机制以及转包裹颗粒的产生和特性。
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本文引用的文献
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Virology. 2012 Oct 10;432(1):29-38. doi: 10.1016/j.virol.2012.05.033. Epub 2012 Jun 22.
2
Mouse hepatic cells support assembly of infectious hepatitis C virus particles.鼠肝细胞支持传染性丙型肝炎病毒颗粒的组装。
Gastroenterology. 2011 Sep;141(3):1057-66. doi: 10.1053/j.gastro.2011.06.010. Epub 2011 Jun 13.
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Distinct functions of NS5A in hepatitis C virus RNA replication uncovered by studies with the NS5A inhibitor BMS-790052.通过研究 NS5A 抑制剂 BMS-790052，揭示了 HCV RNA 复制中 NS5A 的不同功能。
J Virol. 2011 Jul;85(14):7312-20. doi: 10.1128/JVI.00253-11. Epub 2011 May 18.
4
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5
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6
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J Virol. 2010 Feb;84(4):1666-73. doi: 10.1128/JVI.02043-09. Epub 2009 Dec 9.
7
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