分析缺血性脑卒中早期发病患者血清中肽组谱。

Analysis of peptidome profiling of serum from patients with early onset symptoms of ischemic stroke.

机构信息

Department of Biochemistry, Hospital Clínic, Barcelona, Spain.

Department of Biochemistry, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.

出版信息

J Stroke Cerebrovasc Dis. 2014 Feb;23(2):235-40. doi: 10.1016/j.jstrokecerebrovasdis.2013.01.002. Epub 2013 Mar 16.

DOI:10.1016/j.jstrokecerebrovasdis.2013.01.002

PMID:23507461

Abstract

BACKGROUND

We sought to identify new serum biomarkers for the early diagnosis of ischemic stroke.

METHODS

We collected 63 serum samples from patients with neurologic disease (45 patients with ischemic stroke, 18 patients with other neurologic disorders, and 56 healthy controls). Serum peptides were extracted using immobilized copper ion chromatography on a robotic platform. Mass spectra were acquired by matrix-assisted laser desorption/ionization-time of flight mass spectrometry using an Autoflex II spectrometer (Bruker Daltonics, Billerica, MA). Statistical analyses were performed with Clinprotools 2.2 software (Bruker Daltonics) and SPSS software (version 15.0; SPSS, Inc., Chicago, IL).

RESULTS

No peptide biomarker or panel of peptide biomarkers was identified to differentiate between ischemic stroke and other neurologic disease, but ischemic stroke patients were differentiated from healthy controls with a single feature of the peptidome (sensitivity 88.6%; specificity 96.4%).

CONCLUSIONS

Analysis of peptidome profiling of serum could be a useful tool in the search for early diagnostic biomarkers of ischemic stroke.

摘要

背景

我们旨在寻找新的血清生物标志物，用于缺血性脑卒中的早期诊断。

方法

我们收集了 63 例神经疾病患者（45 例缺血性脑卒中患者，18 例其他神经系统疾病患者，和 56 例健康对照者）的血清样本。使用机器人平台上的固定化铜离子亲和层析法提取血清肽。基质辅助激光解吸/电离飞行时间质谱分析采用 Autoflex II 光谱仪（布鲁克·道尔顿公司，比勒陀利亚，MA）进行。采用 Clinprotools 2.2 软件（布鲁克·道尔顿公司）和 SPSS 软件（版本 15.0；SPSS 公司，芝加哥，IL）进行统计分析。