Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
Reprod Biol. 2013 Mar;13(1):100-3. doi: 10.1016/j.repbio.2013.01.166. Epub 2013 Jan 19.
The objective of the study was to examine which cultured endometrial cells are the source and which are the target for lysophosphatidic acid (LPA) in the bovine uterus. LPA concentration as well as mRNA and protein expressions of the enzymes responsible for LPA synthesis (phospholipase A2: PLA2, autotaxin: AX) were greater in epithelial than in stromal cells (P<0.05). In turn, mRNA and protein expression of LPA receptor (LPAR1) was lower in epithelial than in stromal cells (P<0.05). We suggest that LPA in bovine endometrium is produced mainly by epithelial cells and affects mostly stromal cells acting via LPAR1.
本研究旨在探讨牛子宫中哪种培养的子宫内膜细胞是溶血磷脂酸（LPA）的来源，哪种是其靶细胞。LPA 浓度以及参与 LPA 合成的酶（磷脂酶 A2：PLA2、自分泌运动因子：AX）的 mRNA 和蛋白表达在上皮细胞中均高于在基质细胞中（P<0.05）。相反，LPA 受体（LPAR1）的 mRNA 和蛋白表达在上皮细胞中低于在基质细胞中（P<0.05）。我们认为，牛子宫内膜中的 LPA 主要由上皮细胞产生，并通过 LPAR1 主要作用于基质细胞。
