Yamada S
Department of Microbiology, Kawasaki Medical School, Okayama.
Microbiol Immunol. 1990;34(3):221-9. doi: 10.1111/j.1348-0421.1990.tb01005.x.
The effects of immunological IgG binding to Staphylococcus aureus and IgG binding via protein A on the chemiluminescence (CL) response of human phagocytes were examined. The results obtained by enzyme immunoassay showed a clear correlation between the magnitude of the CL response and amount of IgG on protein A-deficient HL-87 strain. Despite no difference in protein A activity between 209P and Cowan I strains, the CL response to IgG-opsonized 209P cells was lower than that to Cowan I cells similarly opsonized. Moreover, the CL response to opsonized HL-87 cells was identical with that of opsonized Cowan I cells, which was a protein A-rich parent strain of the HL-87. The protein A activity of Cowan I cells was significantly decreased when the cells were treated with the Fc fraction of IgG before opsonization, but such a treatment did not change the phagocytic CL response. These results strongly suggest that IgG bound to protein A via its Fc portion has no effect on the phagocytic CL response and that IgG immunologically bound to S. aureus is responsible for the opsonization of the bacteria.
研究了免疫球蛋白G（IgG）与金黄色葡萄球菌结合以及IgG通过蛋白A结合对人吞噬细胞化学发光（CL）反应的影响。酶免疫测定获得的结果显示，在缺乏蛋白A的HL - 87菌株上，CL反应的强度与IgG的量之间存在明显的相关性。尽管209P菌株和考恩I菌株之间的蛋白A活性没有差异，但对IgG调理的209P细胞的CL反应低于对同样调理的考恩I细胞的CL反应。此外，对调理的HL - 87细胞的CL反应与调理的考恩I细胞相同，考恩I细胞是HL - 87的富含蛋白A的亲代菌株。在调理前用IgG的Fc片段处理考恩I细胞时，其蛋白A活性显著降低，但这种处理并未改变吞噬性CL反应。这些结果有力地表明，通过其Fc部分与蛋白A结合的IgG对吞噬性CL反应没有影响，并且与金黄色葡萄球菌免疫结合的IgG是细菌调理作用的原因。
