Tanahashi T, Poulev A, Zenk M H
Lehrstuhl für Pharmazeutische Biologie, Universität München, Federal Republic of Germany.
Planta Med. 1990 Feb;56(1):77-81. doi: 10.1055/s-2006-960889.
A radioimmunoassay for the quantitation of pmol amounts of the therapeutically important Amaryllidaceae alkaloid, galanthamine, has been developed. The antiserum was raised against a conjugate of galanthamine-2-O-hemisuccinate-bovine serum albumin. The measuring range of the assay extends from 0.5 to 100 ng of galanthamine, and as little as 3.5 pmol may be detected. The antiserum is highly specific for galanthamine, showing practically no cross reactivity with different representatives of the most frequently occurring types of alkaloidal structures in Amaryllidaceae. This assay enables a rapid, sensitive and precise quantitation of galanthamine in unpurified plant extracts. The galanthamine concentration in bulbs of several Leucojum aestivum plants and the distribution of galanthamine in some Amaryllidaceae genera of South African origin have been investigated. Preliminary experiments indicate that the galanthamine-specific radioimmunoassay can be a useful tool in medicinal plant breeding as well as in tissue and cell culture studies.
已开发出一种用于定量测定治疗上重要的石蒜科生物碱加兰他敏皮摩尔量的放射免疫分析方法。抗血清是针对加兰他敏-2-O-半琥珀酸酯-牛血清白蛋白偶联物产生的。该分析方法的测量范围为0.5至100纳克加兰他敏，可检测到低至3.5皮摩尔的量。抗血清对加兰他敏具有高度特异性，与石蒜科中最常见的生物碱结构类型的不同代表几乎没有交叉反应。该分析方法能够对未纯化的植物提取物中的加兰他敏进行快速、灵敏和精确的定量。已对几种夏水仙植物鳞茎中的加兰他敏浓度以及一些南非原产石蒜科属中加兰他敏的分布进行了研究。初步实验表明，加兰他敏特异性放射免疫分析方法在药用植物育种以及组织和细胞培养研究中可能是一种有用的工具。
