雄激素受体激动剂促进前脂肪细胞中的成骨基因程序。
Androgen receptor agonism promotes an osteogenic gene program in preadipocytes.
机构信息
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
出版信息
Biochem Biophys Res Commun. 2013 May 3;434(2):357-62. doi: 10.1016/j.bbrc.2013.03.078. Epub 2013 Apr 6.
Abstract
Androgens regulate body composition by interacting with the androgen receptor (AR) to control gene expression in a tissue-specific manner. To identify novel regulatory roles for AR in preadipocytes, we created a 3T3-L1 cell line stably expressing human AR. We found AR expression is required for androgen-mediated inhibition of 3T3-L1 adipogenesis. This inhibition is characterized by decreased lipid accumulation, reduced expression of adipogenic genes, and induction of genes associated with osteoblast differentiation. Collectively, our results suggest androgens promote an osteogenic gene program at the expense of adipocyte differentiation.
摘要
雄激素通过与雄激素受体 (AR) 相互作用来调节身体成分，以组织特异性方式控制基因表达。为了确定 AR 在脂肪细胞前体中的新的调节作用，我们创建了一种稳定表达人 AR 的 3T3-L1 细胞系。我们发现 AR 表达是雄激素介导的抑制 3T3-L1 脂肪生成所必需的。这种抑制的特征是脂质积累减少，脂肪生成基因表达降低，以及与成骨细胞分化相关基因的诱导。总之，我们的结果表明雄激素促进成骨基因程序，而牺牲脂肪细胞分化。
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