东爪哇省玛琅市本土苏云金芽孢杆菌分离株对埃及伊蚊幼虫的毒性研究。
Toxicity studies for indigenous Bacillus thuringiensis isolates from Malang City, East Java on Aedes aegypti larvae.
作者信息
Gama Zulfaidah Penata, Nakagoshi Nobukazu, Setyowati Faridah
机构信息
Graduate School for International Development and Cooperation (IDEC), Hiroshima University, Japan.
出版信息
Asian Pac J Trop Biomed. 2013 Feb;3(2):111-7. doi: 10.1016/S2221-1691(13)60034-9.
OBJECTIVE
To investigate the toxicity of indigenous Bacillus thuringiensis (B. thuringiensis)isolates from Malang City for controlling Aedes aegypti (Ae. aegypti) larvae.
METHODS
Soil samples were taken from Purwantoro and Sawojajar sub-districts. Bacterial isolation was performed using B. thuringiensis selective media. Phenotypic characteristics of the isolates were obtained with the simple matching method. The growth and prevalence of spores were determined by the Total Plate Count method, and toxicity tests were also performed on the third instar larval stage of Ae. aegypti. The percentage of larval mortality was analysed using probit regression. The LC50 was analysed by ANOVA, and the Tukey HSD interval was 95%.
RESULTS
Among the 33 selected bacterial isolates, six were obtained (PWR4-31, PWR4-32, SWJ4-2b, SWJ4-4b, SWJ-4k and SWJ5-1) that had a similar phenotype to reference B. thuringiensis. Based on the dendrogram, all of the bacterial isolates were 71% similar. Three isolates that had a higher prevalence of reference B. thuringiensis were PWR4-32, SWJ4-4b and SW5-1, of which the spore prevalence was 52.44%, 23.59%, 34.46%, respectively. These three indigenous isolates from Malang City successfully killed Ae. aegypti larvae. The PWR4-32 isolates were the most effective at killing the larvae.
CONCLUSIONS
Six indigenous B. thuringiensis isolates among the 33 bacterial isolates found in the Sawojajar and Purwantoro sub-districts were toxic to the third instar larvae of Ae. aegypti. The PWR4-32 isolates were identical to the reference B. thuringiensis and had 88% phenotype similarity. The PWR4-32 isolates had the highest spore prevalence (52.44%), and the early stationary phase occurred at 36 h. The PWR4-32 isolates were the most effective at killing Ae. aegypti larvae (LC50-72 h=2.3×10(8) cells/mL).
目的
研究从玛琅市分离出的本地苏云金芽孢杆菌（B. thuringiensis）菌株对埃及伊蚊（Ae. aegypti）幼虫的毒性。
方法
从Purwantoro和Sawojajar两个分区采集土壤样本。使用苏云金芽孢杆菌选择性培养基进行细菌分离。采用简单匹配法获得分离菌株的表型特征。通过平板计数法测定孢子的生长和流行情况，并对埃及伊蚊的三龄幼虫进行毒性试验。使用概率回归分析幼虫死亡率百分比。通过方差分析分析半数致死浓度（LC50），Tukey HSD区间为95%。
结果
在33株选定的细菌分离物中，获得了6株（PWR4 - 31、PWR4 - 32、SWJ4 - 2b、SWJ4 - 4b、SWJ - 4k和SWJ5 - 1），它们与参考苏云金芽孢杆菌具有相似的表型。基于树状图，所有细菌分离物的相似度为71%。参考苏云金芽孢杆菌流行率较高的三株分离物为PWR4 - 32、SWJ4 - 4b和SW5 - 1，其孢子流行率分别为52.44%、23.59%、34.46%。这三株来自玛琅市的本地分离物成功杀死了埃及伊蚊幼虫。PWR4 - 32分离物在杀死幼虫方面最有效。
结论
在Sawojajar和Purwantoro分区发现的33株细菌分离物中的6株本地苏云金芽孢杆菌分离物对埃及伊蚊的三龄幼虫有毒性。PWR4 - 32分离物与参考苏云金芽孢杆菌相同，表型相似度为88%。PWR4 - 32分离物的孢子流行率最高（52.44%），稳定期早期出现在36小时。PWR4 - 32分离物在杀死埃及伊蚊幼虫方面最有效（72小时半数致死浓度=2.3×10(8) 个细胞/毫升）。
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