表面活性剂对人血清白蛋白原纤维的影响。

Effect of surfactants on preformed fibrils of human serum albumin.

机构信息

Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.

出版信息

Int J Biol Macromol. 2013 Aug;59:39-45. doi: 10.1016/j.ijbiomac.2013.04.014. Epub 2013 Apr 15.

DOI:10.1016/j.ijbiomac.2013.04.014

Abstract

The central reason behind pathogenesis of various neurological disorders is usually attributed to the accumulation of aggregated proteins particularly in fibrillar morphology in vivo. One of the plausible remedial treatments for such disorders may be to identify molecules which are capable of either preventing formation of fibrils or disintegrating formed fibrils. The effect of cationic surfactants cetyl trimethylammonium bromide (CTAB), dodecyl trimethylammonium bromide (DTAB) and the anionic surfactant sodium dodecyl sulfate (SDS) in vitro toward mature HSA fibrils has been investigated. The process has been monitored using ThT fluorescence, FTIR, circular dichroism, fluorescence microscopy and HRTEM. It was observed that the micelles of cationic surfactants were able to effectively disrupt the HSA fibrils, among which CTAB was found to be the most potent.

摘要

各种神经紊乱疾病的发病机制通常归因于聚集蛋白的积累，尤其是体内纤维形态的聚集蛋白。针对此类疾病，一种可行的治疗方法可能是鉴定具有抑制纤维形成或分解已形成纤维能力的分子。本文研究了阳离子表面活性剂十六烷基三甲基溴化铵（CTAB）、十二烷基三甲基溴化铵（DTAB）和阴离子表面活性剂十二烷基硫酸钠（SDS）在体外对成熟 HSA 纤维的影响。采用 ThT 荧光、FTIR、圆二色性、荧光显微镜和 HRTEM 监测该过程。结果表明，阳离子表面活性剂胶束能够有效地破坏 HSA 纤维，其中 CTAB 的效果最强。