利用 DNA 结合荧光染料提高寡核苷酸-纳米颗粒缀合物的认识。
Improving the understanding of oligonucleotide-nanoparticle conjugates using DNA-binding fluorophores.
机构信息
Centre for Molecular Nanometrology, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow G1 1YL, UK.
出版信息
Nanoscale. 2013 May 21;5(10):4166-70. doi: 10.1039/c3nr01197f.
PMID:23598624
Abstract
A novel fluorescent-based method for characterisation of oligonucleotide-nanoparticle conjugates (ONPCs) is reported. We exploit the ability of the double-stranded DNA-binding dye, SYBR Green I, to develop a powerful analytical tool to investigate the melting properties and hybridisation behavior of ONPCs as well as their corresponding DNA-mediated assemblies.
摘要
报道了一种用于寡核苷酸-纳米颗粒缀合物（ONPCs）表征的新型荧光法。我们利用双链 DNA 结合染料 SYBR Green I 的能力，开发了一种强大的分析工具，以研究 ONPCs 及其相应的 DNA 介导的组装体的熔融特性和杂交行为。
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