KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, South Korea.
J Ethnopharmacol. 2013 Jun 21;148(1):99-105. doi: 10.1016/j.jep.2013.03.074. Epub 2013 Apr 16.
Acer tegmentosum, which contains salidroside and tyrosol, has been used for the treatment of hepatic disorders in eastern Asia. However, little is known about its safety.
To determine the safety of Acer tegmentosum, we evaluated its acute oral toxicity and genotoxicity profiles.
Salidroside and tyrosol present in Acer tegmentosum were quantified using high-performance liquid chromatography. Acute oral toxicity testing of Acer tegmentosum was performed in rats. Genotoxicity of Acer tegmentosum was assessed by bacterial reverse mutation, chromosomal aberration, and bone marrow micronucleus tests. All the tests were conducted in accordance with the good laboratory practices.
The amounts of salidroside and tyrosol in Acer tegmentosum were found to be 85.01±1.21mg/g and 3.12±0.04mg/g, respectively. In the bacterial reverse mutation test, Acer tegmentosum increased the number of revertant Salmonella typhimurium TA98 colonies, regardless of metabolic activation by S9 mixture. In contrast, Acer tegmentosum application did not significantly increase the number of chromosomal aberrations in Chinese hamster ovary (CHO)-K1 cells and micronucleated polychromatic erythrocytes in mice. In the acute oral toxicity test, the median lethal dose (LD50) of Acer tegmentosum was found to be >2000mg/kg in rats.
Take together, Acer tegmentosum exhibits mutagenicity, which was evident from the bacterial reverse mutation test. Further studies are needed to identify the components responsible for such an effect and the underlying mechanisms.
青榨槭，含有红景天苷和酪醇，已被用于治疗东亚地区的肝脏疾病。然而，关于其安全性的了解甚少。
为了确定青榨槭的安全性，我们评估了其急性口服毒性和遗传毒性特征。
使用高效液相色谱法对青榨槭中的红景天苷和酪醇进行定量。采用大鼠进行青榨槭急性口服毒性试验。采用细菌回复突变试验、染色体畸变试验和骨髓微核试验评估青榨槭的遗传毒性。所有试验均按照良好实验室规范进行。
青榨槭中红景天苷和酪醇的含量分别为 85.01±1.21mg/g 和 3.12±0.04mg/g。在细菌回复突变试验中，青榨槭增加了鼠伤寒沙门氏菌 TA98 回复突变菌落的数量，而无论 S9 混合物是否存在代谢激活作用。相比之下，青榨槭的应用并未显著增加中国仓鼠卵巢（CHO）-K1 细胞中的染色体畸变数量和小鼠的多染红细胞微核数量。在急性口服毒性试验中，青榨槭在大鼠中的半数致死剂量（LD50）>2000mg/kg。
综合来看，青榨槭具有致突变性，这从细菌回复突变试验中可以明显看出。需要进一步研究以确定导致这种效应的成分和潜在机制。
