大鼠肾上腺中脂质过氧化产物的年龄相关变化。
Age-related changes in lipid peroxidation products in rat adrenal gland.
作者信息
Almeida H, Magalhães M C, Magalhães M M
出版信息
Age (Omaha). 1998 Jul;21(3):119-21. doi: 10.1007/s11357-998-0018-4.
Chloroform-methanol extracts from rat adrenals at five different ages (2, 6, 12, 18 and 24 months), were studied by fluorescence. After obtaining excitation and emission spectra, fluorescence intensity was measured at 365 nm excitation and 455 emission for all time points of aging. An additional study of lipid peroxidation employing a thiobarbituric acid reaction was made. Fluorescence intensity increased during aging from 16.39 × 10(3) arbitrary units of fluorescence per gram of tissue at 2 months, to 34.33 × 10(3) units at 24 months. Thiobarbituric acid reaction products expressed in nmol of malondialdehyde per gram of adrenal increased from 172.97 at 2 months to 640.83 at 24 months. One way analysis of variance revealed a statistically significant difference (p<0.05 and p<0.01 respectively). The results show an age-related steady increase in lipid peroxidation products in rat adrenals and suggest their accumulation in lipofuscin granules.
对五个不同年龄（2、6、12、18和24个月）大鼠肾上腺的氯仿 - 甲醇提取物进行了荧光研究。获得激发光谱和发射光谱后，在衰老的所有时间点测量365nm激发和455nm发射下的荧光强度。还采用硫代巴比妥酸反应对脂质过氧化进行了额外研究。荧光强度在衰老过程中从2个月时每克组织16.39×10³任意荧光单位增加到24个月时的34.33×10³单位。以每克肾上腺中丙二醛的纳摩尔数表示的硫代巴比妥酸反应产物从2个月时的172.97增加到24个月时的640.83。单因素方差分析显示存在统计学显著差异（分别为p<0.05和p<0.01）。结果表明大鼠肾上腺中脂质过氧化产物随年龄稳步增加，并提示它们在脂褐素颗粒中积累。
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