Division of Hypothalamic Research, Department of Internal Medicine, UT Southwestern Medical Center at Dallas, Dallas, TX, USA.
Int J Obes (Lond). 2013 Dec;37(12):1603-7. doi: 10.1038/ijo.2013.48. Epub 2013 Mar 29.
This study investigated the anatomical integrity of the vagal innervation to the gastrointestinal tract following Roux-en-Y gastric bypass (RYGB) in the mouse. Specifically, the surgical procedure was performed in high-fat-fed reporter mice (Phox2b-Cre-tdTomato), in which the entire vagal innervation of the gastrointestinal tract was fluorescently labeled. As a result, our anatomical observations revealed both qualitative and quantitative changes of the vagal supply to the gut after RYGB. This included the extensive denervation of the glandular and distal stomach, and sites of surgical interventions (clipping and anastomosis). Furthermore, the stomach wall after RYGB frequently contained dystrophic axons and endings, suggestive of vagal neurodegeneration. In contrast, RYGB did not significantly modify the innervation to the rest of the intestines and glucostatic organs. In summary, the present study describes a previously unrecognized pattern of vagal remodeling and denervation following RYGB. Our findings may serve as a guideline for future investigations on the role of gut-brain communication in bariatric surgery.
本研究探讨了高脂喂养的报告小鼠（Phox2b-Cre-tdTomato）中 Roux-en-Y 胃旁路（RYGB）术后胃肠道迷走神经支配的解剖完整性。在这些小鼠中，胃肠道的整个迷走神经支配都被荧光标记。因此，我们的解剖观察结果显示，RYGB 后迷走神经对肠道的供应既有定性变化，也有定量变化。这包括腺体和远端胃以及手术干预部位（夹闭和吻合）的广泛去神经支配。此外，RYGB 后的胃壁经常含有营养不良的轴突和末端，提示迷走神经退行性变。相比之下，RYGB 对其余肠道和糖稳态器官的神经支配没有显著改变。总之，本研究描述了 RYGB 后迷走神经重塑和去神经支配的一种先前未被认识到的模式。我们的发现可能为未来关于肠道-大脑通讯在减重手术中的作用的研究提供指导。
