State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Chengguan District, Lanzhou, 730046, People's Republic of China.
Arch Virol. 2013 Oct;158(10):2069-78. doi: 10.1007/s00705-013-1704-9. Epub 2013 Apr 26.
To analyze foot-and-mouth disease virus tropism and host range with respect to the integrin receptor, we cloned cDNAs encoding the integrin αν, β1, β3, β6 and β8 subunits from Chinese yellow cattle and Gansu black swine and carried out comparative analysis of their molecular characteristics. The lengths of the mature proteins and the functional domains of the four integrin β subunits were the same between bovine and swine; however, the number of putative N-linked glycosylation sites and cysteine residues and their arrangement varied. Homology analysis of the nucleotide and amino acid sequences showed that FMDV integrin receptors of Chinese yellow cattle and Gansu black swine are highly conserved. Phylogenetic analysis showed that all FMDV integrin receptor subunits of cattle and swine are clustered into the Artiodactyla group; however, Chinese yellow cattle are phylogenetically closer to sheep than to Gansu black swine. We postulate that the host tropism of FMDV may, in part, be related to the divergence of integrin subunits among different species.
为了分析口蹄疫病毒对整合素受体的嗜性和宿主范围，我们从中国黄牛和甘肃黑猪中克隆了编码整合素αv、β1、β3、β6 和 β8 亚基的 cDNA，并对其分子特征进行了比较分析。牛和猪的四种整合素β亚基的成熟蛋白长度和功能域相同，但潜在的 N 连接糖基化位点和半胱氨酸残基的数量及其排列不同。核苷酸和氨基酸序列的同源性分析表明，中国黄牛和甘肃黑猪的 FMDV 整合素受体高度保守。系统进化分析表明，牛和猪的所有 FMDV 整合素受体亚基均聚类到偶蹄目组中；然而，黄牛在系统进化上与绵羊比与甘肃黑猪更接近。我们推测，FMDV 的宿主嗜性可能部分与不同物种之间整合素亚基的分化有关。
