Ge Yun-Fei, Sun Jun, Jin Chun-Jie, Cao Bo-Qiang, Jiang Zhi-Feng, Shao Jun-Fei
Department of Neurosurgery, Wuxi People's Hospital of Nanjing Medical University, Wuxi, China.
Asian Pac J Cancer Prev. 2013;14(2):963-8. doi: 10.7314/apjcp.2013.14.2.963.
To study the effect of the antagomiR-27a inhibitor on glioblastoma cells.
The miR- 27a expression level in specimens of human glioblastoma and normal human brain tissues excised during decompression for traumatic brain injury was assessed using qRT-PCR; The predicted target gene of miR-27a was screened out through bioinformatics databases, and the predicted gene was verified using genetic report assays; the effect of antagomiR-27a on the invasion and proliferation of glioma cells was analyzed using MTT assays and 5-ethynyl-2'-deoxyuridine (EdU) labeling. A xenograft glioblastoma model in BALB-c nude mice was established to detect the effect of antagomiR-27a on tumour growth.
qRT-PCR results showed that miR-27a significantly increased in specimens from glioblastoma comparing with normal human brain tissues. Th miR-27a inhibitor significantly suppressed invasion and proliferation of glioblastoma cells. FOXO3a was verified as a new target of miR-27a by Western blotting and reporter analyzes. Tumor growth in vivo was suppressed by administration of the miR-27a inhibitor.
MiR-27a may be up-regulated in human glioblastoma, and antagomiR-27a could inhibit the proliferation and invasion ability of glioblastoma cells.
研究抗miR-27a抑制剂对胶质母细胞瘤细胞的作用。
采用qRT-PCR评估在创伤性脑损伤减压手术中切除的人胶质母细胞瘤标本和正常脑组织标本中miR-27a的表达水平；通过生物信息学数据库筛选miR-27a的预测靶基因，并使用基因报告分析验证预测基因；采用MTT法和5-乙炔基-2'-脱氧尿苷（EdU）标记分析抗miR-27a对胶质瘤细胞侵袭和增殖的影响。建立BALB-c裸鼠异种移植胶质母细胞瘤模型，检测抗miR-27a对肿瘤生长的影响。
qRT-PCR结果显示，与正常脑组织相比，胶质母细胞瘤标本中miR-27a显著升高。miR-27a抑制剂显著抑制胶质母细胞瘤细胞的侵袭和增殖。通过蛋白质免疫印迹法和报告基因分析验证FOXO3a是miR-27a的新靶点。给予miR-27a抑制剂可抑制体内肿瘤生长。
miR-27a在人胶质母细胞瘤中可能上调，抗miR-27a可抑制胶质母细胞瘤细胞的增殖和侵袭能力。
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