一种细菌靶向性磁共振造影剂的合成、表征及体外测试
Synthesis, Characterization, and in vitro Testing of a Bacteria-Targeted MR Contrast Agent.
作者信息
Matosziuk Lauren M, Harney Allison S, Macrenaris Keith W, Meade Thomas J
机构信息
Departments of Chemistry, Molecular Biosciences, Neurobiology, Biomedical Engineering, and Radiology, Northwestern University, 2145 Sheridan Rd., Evanston, IL 60208, USA.
出版信息
Eur J Inorg Chem. 2012 Apr;2012(12):2099-2107. doi: 10.1002/ejic.201101362.
Abstract
A bacteria-targeted MR contrast agent, Zn-, consisting of two Zn-dipicolylamine (Zn-dpa) groups conjugated to a Gd chelate has been synthesized and characterized. In vitro studies with and show that Zn- exhibits a significant improvement in bacteria labeling efficiency vs. control. Studies with a structural analogue, Zn-, indicate that removal of one Zn-dpa moiety dramatically reduces the agent's affinity for bacteria. The ability of Zn- to significantly reduce the of labeled vs. unlabeled bacteria, resulting in enhanced MR image contrast, demonstrates its potential for visualizing bacterial infections in vivo.
摘要
一种靶向细菌的磁共振造影剂Zn-已被合成并表征，它由两个与钆螯合物共轭的二吡啶甲胺锌（Zn-dpa）基团组成。对[具体细菌名称1]和[具体细菌名称2]的体外研究表明，与对照相比，Zn-在细菌标记效率上有显著提高。对结构类似物Zn-的研究表明，去除一个Zn-dpa部分会显著降低该造影剂对细菌的亲和力。Zn-能够显著降低标记细菌与未标记细菌之间的[具体参数名称]，从而增强磁共振图像对比度，这证明了它在体内可视化细菌感染的潜力。
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