Center for Bioengineering and School of Electrical and Computer Engineering, University of Oklahoma, Norman, Oklahoma 73019, USA.
Med Phys. 2013 May;40(5):051901. doi: 10.1118/1.4798966.
This paper reports a technique that enables the quantitative determination of the concentration of gold nanoparticles (GNPs) through the accurate detection of their fluorescence radiation in the diagnostic x-ray spectrum.
Experimentally, x-ray fluorescence spectra of 1.9 and 15 nm GNP solutions are measured using an x-ray spectrometer, individually and within chicken breast tissue samples. An optimal combination of excitation and emission filters is determined to segregate the fluorescence spectra at 66.99 and 68.80 keV from the background scattering. A roadmap method is developed that subtracts the scattered radiation (acquired before the insertion of GNP solutions) from the signal radiation acquired after the GNP solutions are inserted.
The methods effectively minimize the background scattering in the spectrum measurements, showing linear relationships between GNP solutions from 0.1% to 10% weight concentration and from 0.1% to 1.0% weight concentration inside a chicken breast tissue sample.
The investigation demonstrated the potential of imaging gold nanoparticles quantitatively in vivo for in-tissue studies, but future studies will be needed to investigate the ability to apply this method to clinical applications.
本文报道了一种通过准确检测诊断 X 射线谱中纳米金颗粒（GNPs）的荧光辐射来定量测定其浓度的技术。
实验中，使用 X 射线光谱仪分别测量了 1.9nm 和 15nmGNPs 溶液的 X 射线荧光光谱，以及鸡胸组织样本中的 X 射线荧光光谱。确定了最佳的激发和发射滤波器组合，以将 66.99keV 和 68.80keV 的荧光光谱与背景散射分离。开发了一种路线图方法，从插入 GNPs 溶液之前获得的散射辐射中减去插入 GNPs 溶液后获得的信号辐射。
该方法有效地将光谱测量中的背景散射最小化，显示了从 0.1%到 10%重量浓度的 GNPs 溶液以及从 0.1%到 1.0%重量浓度的 GNPs 溶液在鸡胸组织样本中的线性关系。
该研究表明，在体内对金纳米颗粒进行定量成像具有潜在的应用前景，但需要进一步的研究来探讨将该方法应用于临床应用的能力。
