Department of Chemistry, University of Sargodha, Sargodha, Pakistan.
Appl Biochem Biotechnol. 2013 Jun;170(4):980-7. doi: 10.1007/s12010-013-0240-0. Epub 2013 May 1.
Gene expression pattern of two important regulatory proteins, salt overly sensitive 2 (SOS2) and plasma membrane protein 3-1 (PMP3-1), involved in ion homeostasis, was analyzed in two salinity-contrasting sunflower (Helianthus annuus L.) lines, Hysun-38 (salt tolerant) and S-278 (moderately salt tolerant). The pattern was studied at selected time intervals (24 h) under 150 mM NaCl treatment. Using reverse transcription PCR, SOS2 gene fragment was obtained from young leaf and root tissues of opposing lines while that for PMP3-1 was obtained only from young root tissues. Both tolerant and moderately tolerant lines showed a gradual increase in SOS2 expression in sunflower root tissues. Leaf tissues showed the gradually increasing pattern of SOS2 expression in tolerant plants as compared to that for moderately tolerant ones that showed a relatively lower level of expression for this gene. We found the highest level of PMP 3-1 expression in the roots of tolerant sunflower line at 6 and 12 h postsalinity treatment. The moderately tolerant line showed higher expression of PMP3-1 at 12 and 24 h after salt treatment. Overall, the expression of genes for both the regulator proteins varied significantly in the two sunflower lines differing in salinity tolerance.
参与离子稳态的两个重要调节蛋白，盐过度敏感 2（SOS2）和质膜蛋白 3-1（PMP3-1）的基因表达模式，在两种耐盐性差异较大的向日葵（Helianthus annuus L.）品系 Hysun-38（耐盐）和 S-278（中度耐盐）中进行了分析。在 150mM NaCl 处理下，选择特定时间间隔（24 小时）进行研究。使用反转录 PCR，从对立品系的幼叶和根组织中获得 SOS2 基因片段，而 PMP3-1 基因片段仅从幼根组织中获得。在向日葵根组织中，两种耐盐和中度耐盐品系的 SOS2 表达均逐渐增加。与中度耐盐品系相比，耐盐植物叶片组织中 SOS2 表达呈逐渐增加的模式，中度耐盐品系的表达水平相对较低。我们发现，在盐处理后 6 和 12 小时，耐盐向日葵品系的根中 PMP3-1 的表达水平最高。中度耐盐品系在盐处理后 12 和 24 小时表现出更高的 PMP3-1 表达。总体而言，两种耐盐性不同的向日葵品系中，两种调节蛋白基因的表达差异显著。
