Sohrab S S, Mandal B, Ali A, Varma A
Advanced Center for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012 India.
Indian J Virol. 2010 Jun;21(1):56-63. doi: 10.1007/s13337-010-0002-3. Epub 2010 Sep 3.
Bottle gourd (Lagenaria siceraria) an important vegetable crop in India was observed to be affected by a chlorotic curly stunt disease (CCSD) during 2003-2006 in the vegetable growing areas of Delhi and adjoining state of Haryana. The affected plants are severely stunted and bear very small chlorotic and mildly curled leaves. Incidence of the disease varied from 4.7 to 36%. The disease could be easily transmitted by whitefly, Bemisia tabaci but not by sap. The causal virus was found to be a Begomovirus on the basis of whitefly transmission and sequence identity of putative coat protein (CP) and replication initiator protein (Rep) genes. The virus was transmitted to Cucumis sativus, Luffa acutangula, L. cylndrica, Lycopersicon esculentum, Nicotiana tabacum and Praecitrullus fistulosus but not to Citrullus lunatus, Cucumis melo, Cucurbita moschata and Vigna unguiculata. The N-terminal 60 amino acids of CP of the virus had 100% sequence identity with all the isolates of Tomato leaf curl New Delhi virus (ToLCNDV) and two isolates of Squash leaf curl China virus (SLCCV). The full length amino acid sequence of the CP and Rep genes had 100% similarity with ToLCNDV-Svr and -Luffa isolates. The phylogenetic analysis showed that the virus associated with CCSD of bottle gourd belongs to ToLCNDV cluster of the begomoviruses. This is the first record of emergence of a Begomovirus associated severe disease in bottle gourd in India.
葫芦（Lagenaria siceraria）是印度一种重要的蔬菜作物，在2003年至2006年期间，德里蔬菜种植区及毗邻的哈里亚纳邦发现其受到一种褪绿卷曲矮化病（CCSD）的影响。受影响的植株严重矮化，叶片非常小，呈褪绿且轻度卷曲状。该病的发病率在4.7%至36%之间。该病害可通过烟粉虱（Bemisia tabaci）轻易传播，但不能通过汁液传播。基于烟粉虱传播以及假定的外壳蛋白（CP）和复制起始蛋白（Rep）基因的序列同一性，发现致病病毒为双生病毒。该病毒可传播至黄瓜（Cucumis sativus）、棱角丝瓜（Luffa acutangula）、普通丝瓜（L. cylndrica）、番茄（Lycopersicon esculentum）、烟草（Nicotiana tabacum）和印度南瓜（Praecitrullus fistulosus），但不能传播至甜瓜（Citrullus lunatus）、甜瓜（Cucumis melo）、南瓜（Cucurbita moschata）和豇豆（Vigna unguiculata）。该病毒CP的N端60个氨基酸与番茄曲叶新德里病毒（ToLCNDV）的所有分离株以及南瓜曲叶中国病毒（SLCCV）的两个分离株具有100%的序列同一性。CP和Rep基因的全长氨基酸序列与ToLCNDV-Svr和丝瓜分离株具有100%的相似性。系统发育分析表明，与葫芦CCSD相关的病毒属于双生病毒的ToLCNDV簇。这是印度首次记录到双生病毒在葫芦上引发严重病害。
