Welman Arkadiusz, Barraclough Jane, Dive Caroline
Cancer Research U.K., Paterson Institute for Cancer Research, University of Manchester, Wilmslow Road, Manchester M20 4BX, United Kingdom.
Transl Oncogenomics. 2007 Mar 28;2:17-33. Print 2007.
The increasing number of proteomic and DNA-microarray studies is continually providing a steady acquisition of data on the molecular abnormalities associated with human tumors. Rapid translation of this accumulating biological information into better diagnostics and more effective cancer therapeutics in the clinic depends on the use of robust function-testing strategies. Such strategies should allow identification of molecular lesions that are essential for the maintenance of the transformed phenotype and enable validation of potential drug-targets. The tetracycline regulated gene expression/ suppression systems (Tet-systems) developed and optimized by bioengineers over recent years seem to be very well suited for the function-testing purposes in cancer research. We review the history and latest improvements in Tet-technology in the context of functional oncogenomics.
蛋白质组学和DNA微阵列研究数量的不断增加，持续稳定地提供了与人类肿瘤相关的分子异常数据。要将这些不断积累的生物学信息迅速转化为临床上更好的诊断方法和更有效的癌症治疗手段，就依赖于使用强大的功能测试策略。此类策略应能识别维持转化表型所必需的分子损伤，并能验证潜在的药物靶点。生物工程师近年来开发并优化的四环素调控基因表达/抑制系统（Tet系统）似乎非常适合癌症研究中的功能测试目的。我们在功能肿瘤基因组学的背景下回顾Tet技术的历史和最新进展。
