[系统性红斑狼疮患者外周血白细胞介素-9及CD4(+)白细胞介素-9(+) T细胞的异常及意义]

[Abnormality and significance of interleukin-9 and CD4(+)interleukin-9(+) T-cells in peripheral blood of patients with systemic lupus erythematosus].

作者信息

Ouyang Han, Shi Yong-bing, Su Nan, Li Ling-yun

机构信息

Department of Nephrology, Second Affiliated Hospital, Soochow University, Suzhou 215004, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2013 Jan 8;93(2):99-103.

PMID:23648344

Abstract

OBJECTIVE

To examine the protein and mRNA levels of interleukin-9(IL-9) and the frequencies of CD4(+)IL-9(+)T-cells in peripheral blood of patients with systemic lupus erythematosus (SLE) and explore the roles of double positive T cells and IL-9 in the pathogenesis of SLE and the effects of glucocorticoid.

METHODS

Twenty-eight hospitalized SLE patients were recruited and 12 healthy volunteers selected as normal controls. The mRNA levels of IL-9 in peripheral blood were measured by real time-polymerase chain reaction (RT-PCR), plasma protein of IL-9 by enzyme-linked immunosorbent assay (ELISA) and frequencies of CD4(+)IL-9(+)T-cells by flow cytometry. And the differences between two groups and the effects of glucocorticoid were analyzed.

RESULTS

(1) The mRNA levels of IL-9 were significantly elevated in SLE patients as compared with normal controls (P < 0.01). The serum levels of IL-9 were significantly higher in active and inactive SLE patients than those in healthy individuals (68 ± 11 vs 26 ± 6 ng/L, P < 0.01; 56 ± 14 vs 26 ± 6 ng/L, P < 0.05). The percentages of CD4(+)IL-9(+)T-cells increased in active SLE patients (1.96% ± 0.31%) versus inactive SLE patients (0.89% ± 0.13%, P < 0.01) and healthy controls (0.28% ± 0.05%, P < 0.001). And it was higher in inactive SLE patients than that in controls (P < 0.05). (2) The serum levels of IL-9 and the frequencies of CD4(+)IL-9(+)T-cells were positively correlated with SLE disease activity index (SLEDAI). (3) The frequencies of CD4(+)IL-9(+)T-cells and the serum levels of IL-9 in 8 untreated active SLE patients decreased at weeks 1, 2 and 3 after the therapy of methylprednisolone (0.8 mg×kg(-1)×d(-1)) versus those at pre-treatment.

CONCLUSION

The abnormalities of IL-9 and CD4(+)IL-9(+)T-cells may play an important role in the pathogenesis of SLE. And the frequencies of CD4(+)IL-9(+)T-cells and the levels of IL-9 are evaluative parameters of SLE activity and severity.

摘要

目的

检测系统性红斑狼疮（SLE）患者外周血中白细胞介素-9（IL-9）的蛋白和mRNA水平以及CD4(+)IL-9(+)T细胞频率，探讨双阳性T细胞和IL-9在SLE发病机制中的作用及糖皮质激素的影响。

方法

招募28例住院SLE患者，并选取12名健康志愿者作为正常对照。采用实时聚合酶链反应（RT-PCR）检测外周血中IL-9的mRNA水平，酶联免疫吸附测定（ELISA）检测IL-9的血浆蛋白水平，流式细胞术检测CD4(+)IL-9(+)T细胞频率。分析两组之间的差异及糖皮质激素的影响。

结果

（1）与正常对照相比，SLE患者IL-9的mRNA水平显著升高（P < 0.01）。活动期和非活动期SLE患者血清IL-9水平均显著高于健康个体（68±11 vs 26±6 ng/L，P < 0.01；56±14 vs 26±6 ng/L，P < 0.05）。活动期SLE患者CD4(+)IL-9(+)T细胞百分比高于非活动期SLE患者（1.96%±0.31% vs 0.89%±0.13%，P < 0.01）和健康对照（0.28%±0.05%，P < 0.001）。且非活动期SLE患者高于对照（P < 0.05）。（2）血清IL-9水平和CD4(+)IL-9(+)T细胞频率与SLE疾病活动指数（SLEDAI）呈正相关。（3）8例未经治疗的活动期SLE患者在甲泼尼龙（0.8 mg×kg(-1)×d(-1)）治疗1、2和3周后，CD4(+)IL-9(+)T细胞频率和血清IL-9水平较治疗前降低。