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使用流式细胞术教授吞噬作用。

Teaching phagocytosis using flow cytometry.

作者信息

Boothby John T, Kibler Ruthann, Rech Sabine, Hicks Robert

机构信息

Departments of Biological Sciences and.

出版信息

Microbiol Educ. 2004 May;5:36-41. doi: 10.1128/jmbe.v5.76.

Abstract

Investigative microbiology on protists in a basic teaching laboratory environment is limited by student skill level, ease of microbial culture and manipulation, instrumentation, and time. The flow cytometer is gaining use as a mainstream instrument in research and clinical laboratories, but has had minimal application in teaching laboratories. Although the cost of a flow cytometer is currently prohibitive for many microbiology teaching environments and the number of trained instructors and teaching materials is limited, in many ways the flow cytometer is an ideal instrument for teaching basic microbiology. We report here on a laboratory module to study phagocytosis in Tetrahymena sp. using flow cytometry in a basic microbiology teaching laboratory. Students and instructors found the flow cytometry data analysis program, Paint-AGate(PRO-TM), to be very intuitive and easy to learn within a short period of time. Assessment of student learning about Tetrahymena sp., phagocytosis, flow cytometry, and investigative microbiology using an inquiry-based format demonstrated an overall positive response from students.

摘要

在基础教学实验室环境中,对原生生物进行微生物学研究受到学生技能水平、微生物培养与操作的难易程度、仪器设备以及时间的限制。流式细胞仪在研究和临床实验室中作为主流仪器的使用越来越广泛,但在教学实验室中的应用却很少。尽管目前流式细胞仪的成本对于许多微生物学教学环境来说过高,且受过培训的教师数量和教学材料有限,但在许多方面,流式细胞仪是教授基础微生物学的理想仪器。我们在此报告一个在基础微生物学教学实验室中使用流式细胞术研究嗜热四膜虫吞噬作用的实验模块。学生和教师发现流式细胞术数据分析程序Paint-AGate(PRO-TM)非常直观,且在短时间内易于学习。使用基于探究的形式对学生关于嗜热四膜虫、吞噬作用、流式细胞术和微生物学研究的学习情况进行评估,结果显示学生总体反应积极。

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