[Effects of oleic acid on expression of adiponectin and its PPARgamma mechanism in 3T3-L1 adipocytes].

OBJECTIVE

To explore the effects of oleic acid on the gene and protein expression of adiponectin and its PPARgamma mechanism in 3T3-l1 adipocytes.

METHOD

L1 adipocytes were incubated with different concentration (25, 50, 100, 200, 400 micromol/L) for 24h, and the mRNA expression of adiponectin and PPARgamma was determined by real-time PCR (relative quantity). And then the 3T3-L1 adipocytes were treated for OA at concentration of 100 micromol/L for 24h with/without the affect of PPARgamma inhibitor GW9662, to determine the gene and protein expression of adiponectin. The protein expression changes of adiponectin were detected by immunoblotting.

RESULT

The mRNA expression of adiponectin and PPARgamma increased at the dosages of 25, 50, and 100micromol /L. With the increase of OA, the mRNA expression of adiponectin and PPARgamma decreased in a dose-dependent manner. Incubating with GW9662 and OA, decreases in the mRNA and protein expression of adiponectin were 77% and 78.01% (P < 0.05), compared with the control respectively.

CONCLUSION

OA could increase the gene expression of adiponectin and PPARgamma mRNA in dose-dependent manner in 3T3-L1 adipocytes,which could be specifically blocked by GW9662, OA indues the expression of adiponectin mRNA and protein in 3T3-L1 adipocytes, possibly by a mechanism involving PPARgamma.