动物大脑在听觉刺激过程中基因活性变化的统计参数映射。

Statistic parametric mapping of changes in gene activity in animal brain during acoustic stimulation.

作者信息

Amelchenko E M, Osokin A A, Zworikina S V, Chekhov S A, Lebedev A E, Voronin P A, Galatenko V V, Vetrov D P, Anokhin K V

机构信息

Laboratory of Neurobiology of Memory, P. K. Anokhin Institute of Normal Physiology, Russian Academy of Medical Sciences, Russia.

出版信息

Bull Exp Biol Med. 2013 Mar;154(5):697-9. doi: 10.1007/s10517-013-2032-5.

DOI:10.1007/s10517-013-2032-5

PMID:23658901

Abstract

We analyzed the expression of transcription factor c-Fos induced by neural activity in the mouse brain after acoustic stimulation. The brain sections of the animals subjected to acoustic stimulation and controls were immunohistochemically stained for c-Fos protein. Statistical parametric mapping (SPM) was used to identify group differences in the acquired images. c-Fos expression was significantly higher in the auditory cortex, amygdala, and hippocampus CA3 area after tone presentation. The proposed combination of SPM with molecular-biological approach to visualization of transcription in the nerve cells makes it possible to identify the collaborative activation of distant brain structures assumed to be the components of united functional systems.

摘要

我们分析了声音刺激后小鼠大脑中神经活动诱导的转录因子c-Fos的表达。对接受声音刺激的动物和对照动物的脑切片进行c-Fos蛋白的免疫组织化学染色。使用统计参数映射（SPM）来识别所获取图像中的组间差异。音调呈现后，听觉皮层、杏仁核和海马CA3区的c-Fos表达显著更高。所提出的将SPM与分子生物学方法相结合以可视化神经细胞转录的方法，使得识别假定为联合功能系统组成部分的远距离脑结构的协同激活成为可能。