Wang Meng-Xi, Li Wen-Lan, Zhang Zheng, Wei Jiang-He, Yang Yun, Xu Yan-Hong, Liang Liang
Drug Research Institute of Life Science and Environment Science, Harbin University of Commerce, Harbin 150076, China.
Zhongguo Zhong Yao Za Zhi. 2013 Jan;38(2):149-53.
The study aimed to clone the open reading frame of chalcone synthase (CHS) from Aquilaria sinensis and analyze the bioinformatics and expression of the gene.
One unique sequence containing CHS domain was discovered in our previous reported wound transcriptome dataset of A. sinensis. The open reading frame of CHS was cloned by RT-PCR strategy with the template of mixed RNA extracted from A. sinensis stem which treated by different wound time. The bioinformatic analysis of this gene and its corresponding protein was performed. The AsCHS1 expression in calli was analyzed with histone gene as an internal control gene under wound condition by qRT-PCR technique.
One unique sequence of CHS, named as AsCHS1, was cloned from A. sinensis. The full length of AsCHS1 cDNA was containing a 1 192 bp ORF that encoded 397 amino acids. The result of qRT-PCR displayed that the highest expression level was at 12 h, which indicated that it was possibly involved in early-stage response to wound.
Cloning and analyzing AsCHS1 gene from A. sinensis provided basic information for study the function and expression regulation of AsCHS1 in the flavonoids biosynthesis.
本研究旨在克隆白木香查尔酮合酶（CHS）的开放阅读框，并分析该基因的生物信息学及表达情况。
在我们之前报道的白木香创伤转录组数据集中发现了一个包含CHS结构域的独特序列。以不同创伤时间处理的白木香茎中提取的混合RNA为模板，采用RT-PCR策略克隆CHS的开放阅读框。对该基因及其相应蛋白质进行生物信息学分析。以组蛋白基因作为内参基因，采用qRT-PCR技术分析创伤条件下愈伤组织中AsCHS1的表达。
从白木香中克隆到一个CHS的独特序列，命名为AsCHS1。AsCHS1 cDNA全长包含一个1192 bp的开放阅读框，编码397个氨基酸。qRT-PCR结果显示，最高表达水平出现在12小时，这表明它可能参与创伤早期反应。
克隆和分析白木香AsCHS1基因，为研究AsCHS1在黄酮类生物合成中的功能及表达调控提供了基础信息。
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