粗糙脉孢菌昼夜节律中的蓝光光感受：黄素三线态参与的证据

Blue light photoreception in Neurospora circadian rhythm: evidence for involvement of the flavin triplet state.

作者信息

Fritz B J, Kasai S, Matsui K

机构信息

Department of Biology, Faculty of Science, Osaka City University, Japan.

出版信息

Photochem Photobiol. 1990 May;51(5):607-10. doi: 10.1111/j.1751-1097.1990.tb01973.x.

DOI:10.1111/j.1751-1097.1990.tb01973.x

PMID:2367558

Abstract

The mechanism of the photoreceptor acting on the circadian conidiation rhythm of Neurospora crassa was studied, with the following results: (1) the efficiency of 8-haloflavins as sensitizers increased with their triplet yields. (2) Phase shifts were not abolished by removal of oxygen prior to illumination. (3) Oxygen inhibited phase shifts when introduced into the cultures after light treatment. It is proposed that the blue light photoreceptor for the circadian clock of Neurospora crassa acts (1) from its triplet state, but (2) not via singlet oxygen; (3) signal transduction involves (an) oxygen-sensitive intermediate(s).

摘要

研究了光感受器作用于粗糙脉孢菌昼夜分生孢子形成节律的机制，结果如下：（1）8-卤代黄素作为敏化剂的效率随其三线态产率增加。（2）在光照前去除氧气并没有消除相移。（3）在光照处理后将氧气引入培养物中时，氧气会抑制相移。有人提出，粗糙脉孢菌生物钟的蓝光光感受器（1）从其三线态起作用，但（2）不通过单线态氧；（3）信号转导涉及对氧敏感的中间体。