西尼罗河病毒复制子表达分泌型海肾荧光素酶的构建及鉴定。
Development and characterization of West Nile virus replicon expressing secreted Gaussia luciferase.
机构信息
Center for Emerging Infectious Diseases, State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
出版信息
Virol Sin. 2013 Jun;28(3):161-6. doi: 10.1007/s12250-013-3332-7. Epub 2013 May 25.
Abstract
We developed a Gaussia luciferase (Gluc) reporter replicon of West Nile virus (WNV) and used it to quantify viral translation and RNA replication. The advantage of the Gluc replicon is that Gaussia luciferase is secreted into the culture medium from cells transfected with Gluc replicon RNA, and the medium can be assayed directly for luciferase activity. Using a known Flavivirus inhibitor (NITD008), we demonstrated that the Gluc-WNV replicon could be used for antiviral screening. The Gluc-WNV-Rep will be useful for research in antiviral drug development programs, as well as for studying viral replication and pathogenesis of WNV.
摘要
我们构建了西尼罗河病毒（WNV）的 Gaussia 荧光素酶（Gluc）报告子复制子，并利用它来定量分析病毒的翻译和 RNA 复制。Gluc 复制子的优势在于，转染 Gluc 复制子 RNA 的细胞会将 Gaussia 荧光素酶分泌到培养基中，并且可以直接从培养基中检测荧光素酶的活性。利用一种已知的黄病毒抑制剂（NITD008），我们证明 Gluc-WNV 复制子可用于抗病毒筛选。Gluc-WNV-Rep 将有助于抗病毒药物开发计划中的研究，以及研究 WNV 的复制和发病机制。
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