Musara Colin, Vaillant Camille
Department of Preclinical Veterinary Studies, University of Zimbabwe.
Onderstepoort J Vet Res. 2013 Mar 5;80(1):518. doi: 10.4102/ojvr.v80i1.518.
The distribution of interstitial cells of Cajal (ICC), the probable pacemakers in gastrointestinal motility, was investigated using an antigenic marker of gastric ICC known as C-Kit. Antiserum raised against the general neuronal marker protein gene peptide 9.5 (PGP) as well as the nitrergic neuronal marker neuronal nitric oxide synthase (nNOS) were used to investigate the distribution of gastric nerves. Polyclonal goat anti-human C-Kit was reliable in labelling ICC in the stomach. Two classes of ICC were identified according to their distribution: ICC-MY distributed around the periphery of myenteric ganglia and ICC-IM in the circular and longitudinal muscle layers. The neuronal marker PGP was reliably consistent in revealing the density and distribution of the enteric nervous system. Density of nerve fibres was higher in circular smooth muscle than in longitudinal smooth muscle. From nNOS immunohistochemistry, it is evident that inhibitory (nitrergic) nerves constitute a substantial fraction of the enteric nervous system.
使用一种名为C-Kit的胃ICC抗原标记物，对胃肠道运动中可能的起搏细胞——Cajal间质细胞（ICC）的分布进行了研究。用针对一般神经元标记物蛋白基因肽9.5（PGP）以及含氮能神经元标记物神经元型一氧化氮合酶（nNOS）产生的抗血清来研究胃神经的分布。多克隆山羊抗人C-Kit在标记胃中的ICC方面是可靠的。根据其分布确定了两类ICC：肌间神经丛ICC（ICC-MY）分布在肌间神经节周围，以及环形和纵行肌层中的肌内ICC（ICC-IM）。神经元标记物PGP在揭示肠神经系统的密度和分布方面可靠且一致。环形平滑肌中的神经纤维密度高于纵行平滑肌。从nNOS免疫组织化学来看，显然抑制性（含氮能）神经构成了肠神经系统的很大一部分。
